| ObjectiveBy using artificial ligament made by silk to reconstruct ACL of animal,the paper aims at studying the integration between silk artificial ligament and bone interface of the animals for experiment by methods of gross observation, histology, imageology, and biomechanics, what's more,to acknowledge the specific transferring process of the interface by the entities and statistics. It lays a solid foundation for localization research of silk artificial ligament.MethodsExperimental group and the control group were used as a silk artificial ligament reconstruction materials, the establishment of 36 adult New Zealand white rabbits white rabbits with unilateral anterior cruciate ligament reconstruction model. Experimental group before the silk artificial ligament soaked in PBS solution containing BMP 0.5mg/ml of four hours in the control group received only PBS solution, pre-wet for 4 hours. Simulate the clinical ACL reconstruction, ligament bone tunnel at both ends by some who were supporting phalanx steel compression screw fixation of cancellous bone. Were drawn after the 4,8,16 weeks for gross observation, histological, radiological and biomechanical examination. Statistical analysis of experimental data obtained.Results4 weeks after the control group had no significant ligament synovial coverage, the interface shows a lot of loose connective tissue filled with inflammatory cells infiltrates, CT images showed Iigament-a clear demarcation between the bone; significantly after 8 weeks ligament synovial covering, the interface shows dense fibrous tissue closely connected with the ligament, new bone formation, CT images showed ligament-bone boundaries between the obvious; after 16 weeks were synovial ligament basic package, the interface shows that Sharpey fiber structure, CT images showed ligament-close connection between the bone.4 weeks after the experimental group had significant synovial ligament that covers the interface is full of a lot of dense connective tissue, inflammatory cell infiltration, and new bone formation, CT images showed ligament-bone interface boundaries between the obvious; 8 weeks after ligament synovial basically wrapped the interface part of the area is the Sharpey fiber structure, significantly reduced inflammatory cells, CT images showed ligament-no obvious boundaries between bone interface; after 16 weeks of synovial ligament was completely wrapped, similar to the shape of autologous ACL, ligament-Sharpey fiber bone interface to the main structure, CT images showed ligament-bone interface connection close.All experimental animals were selected 4,8,16 weeks after ligament-bone interface CT value check, and then use image processing software eflime The data were statistically analyzed. The results showed significant differences (P <0.05).Silk ligament in vitro mechanical tests, the data were higher than autogenous ACL, biomechanical testing after different time points were found in the ligament from the bone tunnel to pull out, no one ligament rupture. Statistically significant difference between the two groups (P<0.05).ConclusionTendon—bone interface of ACL reconstruction by silk artificial ligament can indirect connected by each other with structure of Sharpey fibers, and the cytokines BMP can promote tendon—bone interface healing, which also enhance its healing strength.SignificanceAs people's living level improves and masses sports develops, articular ligament injuries and tendon injuries of limbs are inclined to increase. Many ligament reconstruction and transplanting operations greatly promote the development of ligament subsitude material. Especially from 1970s on, some artificial ligament products come into market, which accelerate basic researches and clinical practices. Artificial ligament has the following advantages:for the area to avoid the complications of surgical trauma,and to prevent the spread of disease allogeneic tendon,to shorten the surgery and patient recovery time, after the source of widespread mass production. LARS artificial ligament has incommensurable advantages. Nowadays, cases of reconstructing ligament of all body parts present fast growth the tendency, what's more, LARS artificial ligament we are using now is expensively provided abroad. Therefore, the basis of comparative study to find a perfect material to make artificial ligament, apply as soon as possible to the animal and clinical trials, lower prices, better operation, with independent intellectual property rights is the starting point of the research.The silk artificial ligament (rabbit-to-use),which in the experimental applied,is designed of our reach group, manufacturing commissioned the Institute of Materials Science and Engineering college, Donghua University. Intra-articular part of silk ligament is loose parts, dense part in the bone tunnel, and both ends of the guideline long enough so that operation time was shortened. Ligament surgery applied at both ends of the fixed screw extrusion technology, to avoid the animals brought in suspension fixed wiper effect, try to simulate the process of clinical surgery. Used its characteristic of absoring and releasing drugs absorption characteristics, silk artificial ligament combined with bone morphogenetic protein-2 (BMP-2),which cytokine can promote tendon healing This study in the materials research of knee ligament reconstruction is at international advanced level.Silk artificial ligament enhanced by BMP if successfully developed, not only for research in the field of artificial ligaments in China is a huge boost, but also will have huge economic and social benefits. Let our patients,who need ACL reconstruction,can actually use the artificial ligament of proprietary intellectual property rights is our persistent goal. |
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