Study On The Optimal Administrative Time Of Granulocyte-colony Stimulationg Factor For Mobilizing Marrow Stem Cell To Treat Ischemic Cerebral Infarction In Rats

[Objective]:To investigate the optimal administrative time of granulocyte-colony stimulating factor for mobilizing marrow stem cell to treat ischemic cerebral infarction in rats.[Methods]:Twenty-four hours after having successfully ligated the left middle cerebral artery (MCA) and left common carotid arteries (CCAs) to induce cerebral infarction, Zeal Longa's neurological behavioral assessments were performed. Forty rats with score equal to or mare than 2 were randomly divided into four groups, each group 10 rats. The acute-phase administration group:to be treated with abdominal cavity injection of G-CSF 10μg/kg/day for 10 consecutive days (days 1 to 10); The subacute-phase administration group:to be treated with abdominal cavity injection of G-CSF 10μg/kg/day for 10 consecutive days (days 11 to 20); The acute-phase control group:be treated daily with abdominal cavity injection of comparative voluminal saline for 10 consecutive days (days 1 to 10);The subacute-phase control group:to be treated daily with abdominal cavity injection of comparative voluminal saline for 10 consecutive days (days 11 to 20). Zeal Longa's neurological behavioral assessments, body weight measurements and rates of body weight loss were performed on the day 1,10, 20 and 27 subsequent to MCA ligation. On the 27th day subsequent to cerebral infarction, the rats were killed and their cerebrums were taken out and stained by TTC and HE to detect the volume of infarction and pathological change.[RESULT]:Before and on the 10th day subsequent to middle cerebral artery occlusion (MCAO), neurological behavioral assessments and rates of body weight loss were not significantly difference among the four groups. On the 27th day subsequent to MCAO, the rate of body weight loss of the acute-phase administration group was much better than that of the other three groups; the rate of body weight loss of the subacute-phase administration group was better than that of the two control groups; there was no difference between the two control groups. On the day 20 and 27 subsequent to MCAO, the neurological behavioral assessment of the acute-phase administration group was smaller than that of the other three groups; there was no difference among the other three groups. On the 27th day, the ratio of the infarction volume to total cerebral volume of the acute-phase administration group was smaller than that of the other three groups; the ratio of the infarction volume to total cerebral volume of the subacute-phase administration group was smaller than that of the two control groups; there was no difference between the two control groups. There was neovascularization with growth of new vessels in the area of ischemia in the acute-phase administration group. That were not observed in the other three groups.[Conclusion]:The acute phase of cerebral infarction is the optimal administrative time of Granulocyte-colony stimulating factor for mobilizing marrow stem cell to treat ischemic cerebral infarction.