Protective Efficacy Of Co-immunization With Recombinant Protein Rsj14-3-3 And Rsj26gst Vaccine Against Schistosoma Japonicumin Mice

Objective To study the protective efficacy of co-immunization with recombinant Sj14-3-3 (rSj14-3-3) and recombinant Sj26 protein (rSj26GST) vaccine against Schistosom a japonicum in BALB/c mice, and to study the anti-schistosomiasis effect in mice immunized with co-immunized vaccine group.Methods The E. coli BL21/p ET28a of Sj14-3-3 gene and Sj26GST gene was LB/Kana/IPTG/X-gal pan, Culture, Collection of bacteria, ultrasound broken bacteria,, purified, Purified proteins were collected from 5μl to SDS-PAGE, purified observed results. Bradford determination of recombinant protein rSj14-3-3 and rSj26 GST concentration. To 100 6 to 8-week-old BALB/c female mice were randomly divided into A, B, C, D, E5 group, n =20. A 2耀oup (infection control group), in clause 0 weeks of each mouse by subcutaneous injection of 50μl PBS and the multi-point equal volume of Freund's complete adjuvant (CFA), in paragraph 2 and 4 weeks of each mouse subcutaneous injection of 50μl PBS to strengthen multi-point increase an equal volume of Freund's incomplete adjuvant (IFA). B group (rSj14-3-3 group), each rat in the first 0, 2, 4 weeks after subcutaneous 100μg (50μl) rSj14-3-3. C group (rSj26 GST group), each rat in the first 0,2,4 weeks after subcutaneous 100μg (50μl) rSj26 GST. D group (rSj14-3-3+rSj26GST combined immunodeficiency group), in paragraph 0,2,4 weeks after subcutaneous injection of multi-point rSj14-3-3 and rSj26 GST of 50μg.Two weeks after last immunization, each mouse was challenged with 30±2 cercariae of S. japonicum Chinese strain. E group (control group) did not make any deal. At the 45th day post-infection, mice were sacrificed and the worms were perfused from portal vein and the number of worms and eggs in liver tissue were counted; he size of single egg granulomas in livers was measured with micrometer.The level of hyaluronic acid (HA) and laminin (LN) in sera of the mice was determined by ELISA.Results The worm reduction rate in co-immunized group was 38.4%, significantly higher than that in rSj14-3-3 group (19.8%,P< 0.05) and rSj26 GST group (21.6%,P<0.05). Liver egg reduction rate in co-immunized group, rSj14-3-3 group and rSj26GST group were 48.8%, 35.3% and 41.4% respectively. The number of eggs female in liver of co-immunized mice and rSj26 GST group were significantly higher than that in control group(P<0.01).The mean hepatic egg granuloma diameters of the co-immunized vaccine group(173.9±35.0um) was significantly smaller than that in infection control group (267.7±28.6um) (P<0.01). The level of HA and LN in sera of co-immunized vaccine vaccinated mice was markedly lower than in infection control group (P<0.01).Conclusion Compared to the immunization with rSj14-3-3or rSj26 GST alone, the co-immunization with rSj14-3-3 and Sj26 GST can enhance protective efficacy in BALB/c mice. The results showed that co-immunized vaccine may act as an effective inhibitor against formation of egg granuloma and reduce immunopathological damage caused by Schistosoma japonicum in the host.