Mir-214 Modulates Proliferation By Targeting Mek3 And Jnk1 In Cervix Cancer Cell

[Objective]MicroRNAs (miRNAs) have emerged relatively as a new class of small, non-coding RNAs of 18 to 25 nucleotides that regulate gene expression. miRNAs has implicated in tumor genesis, development and cell apoptosis. Here we identified miR-214 can accelerate tumour genesis and apoptosis.[Methods]First, the total RNA of human cervix cancer tissue and cervix tissue was analyzed by miRNA expression profile and Northern blotting, RNA was isolated with Trizol reagent. We found that miR-214 expresses at a low level in cervix cancer tissue than that in cervix tissue. Then The endogenous miR-214 was blocked in human cervix cancer cell line HeLa and the changes of cell phenotypes were detected with MTT assay and colony formation assay.【Results】1.MiR-214 expresses at a low level in cervix cancer tissue than that in cervix tissue.2.we identified the candidate target genes MEK3 and JNK1 for miR-214.3.MiR-214 accelerates the tumour genesis and apoptosis of cervix cancer cell line mediate by MEK3 and JNK1, two members of MAP kinase pathway.【Conclusion】Our results indicate that in HeLa cell, over-expressed miR-214 can directly down-regulate the expression level of MEK3 and JNK1, two members of MAP kinase pathway, leading to the activation of cell apoptosis and inhibition of cell proliferation.