The Experiment Of The Effects Of The Xinshenyihao On Chronic Stress On Serum-induced Sod,mda And C-fos,caspase-3 In Rat Colon Intervention

ObjectiveInvestigate the therapeutic effects of the xinshenyihao on chronic psychological stress on serum-induced oxidative and damage in rat colon intervention. Explore the psychological stress of chronic rat model of colon injury in rats mechanism.Methods1. This experiment used 48 male SD rats.They were divided into 6 groups by random (8 per group).Divided into blank control group, model, western medicine group(WM) (paroxetine), on the xinshenyihao low, middle and high dose group.2. Chronic psychological stress model:at the beginning of every morning, the folder tail (1 hour), drilling barrels (2-3 hours), fixed limbs (2 hours), water (24 hours), cut fresh (24 hours) per day above a stimulating way, alternating 15 days. Treatment groups, the WM group will be prepared in accordance with 0.2mg/ml suspension paroxetine weight given lml/100g; medicine low, middle and high 2ml/100g weight given to each group (about 0.15g,0.30g,0.60g/100g crude drug). Control group given an equivalent model with distilled water gavage, continuing for 15 days.3. Drawn:the femoral artery and bled to death Blood 5ml, to 3000r/min centrifugal 10min, separation of serum, in strict accordance with operation instructions SOD activity and MDA content. At the same time, all rats anatomy, ventral incision line from the colon preserved in 10% formalin solution, conventional paraffin-embedded, biopsy, HE stain, use 16D eyepiece micrometer Net (an area of 0.1024mm) at 400 times the light microscope colonic mucosa under inflammatory cells count the number of counts per slice 5 grid averaging the results per mm2 containing inflammatory cells express. By immunohistochemical detection of colon c-fos, caspase-3 protein expression.4. We analyzed the data by analysis of variance and q-test.Results1.Chronic psychological stress model:before the experiment all animals in good condition, after the experiment, the control group had no significant abnormal performance,with the model with time, rats showed a gradual reduction in appetite, weight gain slow, color yellow animals and symptoms such as mental depression. Air-line with the macroeconomic performance of disorders card.2.Colonic mucosa of rats in each group pathological situations:with the control group compared to rats of model group increased the number of colonic mucosa cells (P <0.05),compared with the model group, the treatment group lymphocytes reduced(P<0.05), with WM group, the physical and mental high-dose group on the xinshenlhao reduced in the number of lymphocytes (P<0.05).3.Model group, serum SOD activity than the control group(P<0.05),xinshenyihao group, WM, serum SOD activity were higher than the model group(P<0.05).With the WM group, on xinshenyihao SOD activity in the lower dose group (P<0.05). Compared with blank control group, model group, serum MDA content increased(P<0.05), compared with the model group, on the xinshenyihao serum MDA levels were lower (P<0.05), with WM group comparison, on xinshenyihao MDA content was the lower group (P<0.05).4.Blank control group, colonic mucosa layer, myenteric plexus of intestinal c-fos gene expression were negative, and the model have different levels of positive expression in the mucosal layer model group and the comparison between the control group(P<0.05),its expression is higher than the control group.Compared with model group, the treatment group the expression of c-fos-positive reduced(P<0.001). xinshenyihao low-medium-dose group the expression of c-fos-positive than the WM group(P<0.05).In the intestinal myenteric plexus model group compared with control group the difference was significant (P<0.05), its expression should be higher.Similarly, compared with the model group, the treatment group the expression of c-fos-positive reduction(P<0.001), and the dose on xinshenyihao group and WM there is no difference between groups (P>0.05).5.caspase-3 protein expression in the model group is higher than the control group (P<0.05),but expression of all the the xinshenyihao groups, WM group is below the model group (P<0.05), compared with the WM group, the expression of low-, Middle dose group is higher (P<0.05).Conclusions1. The psychological stress of chronic rat model is approximate to be in line with the symptom of Qiji disorder macro show. 2. Psychosomatic medicine paroxetine tablets and the xinshenyihao on the chronic psychological stress model of rat colon injury has a very good protective effect and xinshenyihao effects of high dose group is superior to WM group.3. xinshenyihao has reduced serum MDA content, SOD activity increased to improve the antioxidant capacity in rats, reducing lipid peroxidation injury in rats, its effect is superior to WM group.4. Chronic psychological stress model of rat colon c-fos, caspase-3 expression have a significant change, suggesting their involvement in stress-induced formation of colon injury, xinshenyihao, Paroxetine reduced the colon can be c-fos, caspase-3 expression.