The Variation And Clinical Significance Of Cd₄⁺cd₂₅⁺ Regulatory T Cell In Pre/post-operation Peripheral Blood Of Gastric Cancer

Objective:To investigate the variation of CD4+CD25+regulatory T cell (Treg) in peripheral blood of gastric cancer, and explore the effects of Treg on tumor immunity.Methods:Peripheral blood were collected from 38 patients with gastric cancer on the 3th day before operation and the 14th day after operation, as control, peripheral blood from 20 healthy adults were also collected. Flowing cytometry were used to analyze CD4+CD25+Treg, CD4+T and CD8+T cells. The results which combinded with clinical and pathological datum were statistically analysed.Results:1. In peripheral blood of patients with gastric cancer, the percentage of Tregs in pre-operation was significantly higher than that of the control (P<0.01), the percentage of Treg in post-operation had no significant difference with the control (P>0.05). The percentage of Tregs in pre-operative peripheral blood of patients with gastric cancer was significantly higher than that of post-operation (P＜0.01).2. In pre-operative peripheral blood of patients with gastric cancer, the percentage of CD4+T cells in pre-operation had no significant difference with the control (.P>0.05), the percentage of CD4+T cells in post-operation had no significant difference with the control (P>0.05).The percentage of CD4+T cells in post-operative peripheral blood of patients with gastric cancer had no significant difference with that in pre-operation (P＞0.05)3. In patients with gastric cancer, the percentage of CD8+T cells in pre-operative peripheral blood was significantly lower than that in the control (P<0.01), the percentage of CD8+T cells in post-operation had no significant difference with the control (P>0.05). The percentage of CD8+T cells in post-operative peripheral blood was significantly higher than that in pre-operation (P<0.01). The percentage of CD8+T cells were negatively correlated with the percentage of Tregs in pre-operation peripheral blood (P<0.05)4. In pre-operative peripheral blood of patients with gastric cancer, the percentage of Tregs in different status of lymph node metastasis and TNM staging had significant difference (P<0.05), the percentage of Tregs in pre-operation was positively correlative with lymphatic metastasis and TNM staging (PO.Ol), but there were no correlation with depth of invastion, metastasis, degree of differentiation or histopathological type of the tumor (,P>0.05)5. In patients with gastric cancer, the decrease of CD4+CD25+Tregs between post-operation and pre-operation had no correlationship with depth of invasion, lymphatic metastasis, metastasis, TNM staging, degree of differentiation, histopathological type or operative approach of the tumor (P>0.05)Conclusion:1. The percentage of Tregs in peripheral blood increases significantly in patients with gastric of cancer, which indicates that they maybe one of the mechanism of immuno-inhibition in the patients with malignance. We can relieve the tumor induced immune inhibition and improve the effect of tumor treatment by efficiently control the function and quantity of these cells.2. The percentage of CD8+T cells in peripheral blood decreases significantly in patients with gastric of cancer, and the percentage of CDg+T cells in pre-operation peripheral blood negatively correlated with the percentage of Tregs,which indicates that Tregs promote tumor cell growth and development by inhibiting CDg+T cell proliferation and function.3. The percentage of Tregs in peripheral blood of patients with gastric cancer is correlative with lymphatic metastasis and TNM staging, which suggests that Tregs is related to the invasiveness and lymphatic metastasis of tumor, and Treg can be used to detect the disease progression as the immunological indicator.4. The percentage of Tregs in post-operative peripheral blood of patients with gastric cance was lower than that in pre-peration, and the value of decrease of Tregsr has no correlationship with depth of invasion, lymphatic metastasis, metastasis, TNM staging, degree of differentiation, histopathological type or operative approach of the tumor, which suggest that the resection of tumor mass can make the proportion of Tregs decreased in peripheral blood, and the consequence is improving the body's anti-tumor immunity.