Expression And Significance Of Hsp60 And Nm23 Protein In Colon Carcinoma

Colon cancer (colon carcinoma) is a common malignant tumor of the digestive tract, which can occur in any part of the colon. The incidence of colon carcinoma in different parts of the world are quite different. In the economically developed countries, such as North America, Oceania, Western Europe and other countries the incidence is particularly high, and lower in Asian and African regions. In southern China, especially along the southeast coast, the incidence of colon carcinoma is significantly higher than the north. It has been rising on the world in the past 20 years. China is no exception, especially in large cities. The present study results show that the occurrence and development of colon carcinoma is a multi-factor, multi-stage, multi-genes involved in genetic disease.Heat shock proteins (HSPs) are a group of highly conserved proteins that reside extensively in both prokaryotic and eukaryotic cells. HSPs mainly act as molecular chaperones which involve in protein synthesizing and folding. In addition, a number of studies showed they played a role in the occurrence and development of cancer. HSP60 is a member of the heat shock proteins family, which was wined much attention. Nm23 is an anti-metastatic oncogene, which participates in the invasive and metastatic course and the developmental course.It's high expression show low(?). As a tumor metastasis suppressor gene, it is the most eye-catching. But it still had the very difference of its significance in the colon cancer. In the present study, we detected the expression of HSP60 and nm23 protein in colon carcinoma, hyperplasia adjacent to the tumor and normal colonic mucosa tissue, and seek their clinicopathological significances in colon carcinoma, as well as the relationship of HSP60, nm23 protein's expression in colon carcinoma.Materials and Methods1.All the specimens came from the First Affiliated Hospital of Zhengzhou University in the year from 2007 to 2008. Patients before did not receive radiotherapy and chemotherapy and other treatment for other tumors. the expression of HSP60 and nm23 protein were detected in 60 cases of colon carcinoma,45 cases of atypical hyperplasia tissues and 21 cases of normal colon mucosa respectively2.Statistical analysis:All the data were analyzed by SPSS 16.0 statistical package. The Chi-square was used in the comparison of positive rates; the relation of two variables was analyzed by the spearman level correlation analysis. The level of significant difference wasα=0.05Results1. The positive expression rates of HSP60 in colon normal carcinoma tissues, atypical were 78.3%(47/60),37.8%(17/45) and 20.0%(4/20) respectively; the positive expression rate of HSP60 in the carcinoma tissues was compared with that in the atypical hyperplasia tissues and normal mucosa tissues from each other, and there was statistical significance (P<0.01). And there was no significant difference in the expression of HSP60 in hyperplasia adjacent to the tumor and normal colon mucosa (P> 0.05).2. There was no relationship between the expression of HSP60 and the patient's sex, age and tumor differentiation, depth of invasion (P>0.05). But the expression of HSP60 in colon carcinoma with lymph node metastasis 92.0%(13/25) was si(?)antly high than those without 68.6%(24/35) (P<0.05)3. Th(?)sitive expression rates of nm23 protein in colon normal carcinoma tissues, aty (?) hyperplasia adjacent to the tumor and normal colon mucosa were 5(?)4/60),83.2%(37/45) and 91.7%(18/20) respectively; and the positive c(?)on rate of nm23 protein in the carcinoma tissues was compared with that i(?)typical hyperplasia tissues and normal mucosa tissues from each other, tl(?) was statistical significance (P<0.01). And there was no significant di(?)nce in the expression of nm23 protein in hyperplasia adjacent to the tumor a(?)mal colon mucosa (P> 0.05).4 Th(?) no correlation between the expression of nm23 protein and the patient's sex, age amd tumor differentiation (P>0.05); but a relationship was found between the n(?) protein expression and the tumor invasion depth and lymph node metastasis (P<0.05), with the invasion depth and lymph node metastasis increased its expression is decreasing.5. The relativity of the expression of HSP60 and nm23 protein in colon carcinoma was analyzed statistically. Results showed that the expression of HSP60 was not correlated with that of nm23 protein in colon carcinoma (P<0.05).Conclusions1. The significantly higher expression of HSP60 was observed in colon carcinoma as compared with that in atypical hyperplasia adjacent to the tumor and normal colon mucosa. And its expression was correlated with the lymph node metastasis, suggesting the invasive and metastasis ability of the tumor was enhanced, when the expression of HSP60 up.2. The significantly lower expression of nm23 protein was observed in colon cinoma as compared with that in atypical hyperplasia adjacent to the tumor and (?) colon mucosa. And its expression was correlated with tumor invasion depth and lymph node metastasis, suggesting the invasive and metastasis ability of the tumor was enhanced, when the expression of HSP60 fall.3. There was no correlation between the expression of HSP60 and nm23 protein in the colon carcinoma.4. Combined detection of the two indexes may be usefully for the prognostic judgment of colon cancer.