Changes And Their Clinical Significance Of Ifn-γ,il-10 And S-100b Protein Content In Cerebrospinal Fluid And Serum Of Patients With Central Nervous System Infections

Objective To observe changes of IFN-γ,IL-10 and S-100B protein level in cerebrospinal fluid and serum in patients with viral meningoencephalitis and purulent meningoencephalitis in the acute and the recovery phase, and to explore it significance.Methods 32 cases of patients with viral meningoencephalitis (VM group) and 28 cases of patients with purulent meningoencephalitis (PM group) were collected as the observed group, and 30 cases of patients with no nervous system organic disease were collected as the control group. IFN-γ,IL-10 and S-100B protein concentration in cerebrospinal fluid and serum were detected by Double-antibody sandwich ELISA. Statistical analysis was performed by SPSS14.0 statistical package.Results (1) VM group and PM group in the acute phase have significantly higher concentration of IFN-γ,IL-10 and S-100B protein in cerebrospinal fluid and serum than in the recovery phase and the control group(P<0.01). (2) VM group has significantly higher concentration of IFN-γin cerebrospinal fluid and serum than PM group in the acute phase (P<0.01). PM group has significantly higher concentration of IL-10 and S-100B protein in cerebrospinal fluid and serum than VM group in the acute phase(P<0.01). (3) Ratio of IFN-γ/IL-10: VM> the control group > PM (P<0.01). (4) The coma patients have significantly higher concentration of S-100B protein than no coma patients in the acute phase (P<0.01). (5) S-100B protein concentration in serum of VM and PM group in the acute phase is significantly correlated with S-100B protein concentration in CSF (r=0.949, P<0.01). (6) S-100B protein concentration in CSF of VM in acute phase is significantly positively correlated with IFN-γconcentration (r=0.557, P<0.05), S-100B protein is not significantly correlated with IL-10 (r=0.057, P>0.05); S-100B protein concentration in CSF of PM in acute phase is significantly positively correlated with IL-10 concentration (r=0.463, P<0.05), S-100B protein is not significantly correlated with IFN-γ(r=0.189, P>0.05);IFN-γis not significantly correlated with IL-10 in both groups (P>0.05).Conclusion S-100B protein concentration in cerebrospinal fluid and serum reflects the extent of damage of glial cells. Detection of IFN-γ,IL-10 in cerebrospinal fluid or serum and the ratio of IFN-γ/IL-10 can help identify viral meningoencephalitis and purulent meningoencephalitis diagnosis. The imbalance of IFN-γand IL-10 may increase neural immunity injury in the central nervous system infections.