Sirna Blocking Wnt / β-catenin Signaling Pathway Affect Invasive Ability In Endometrial Eutopic Endometrial Stromal Cell

Objectiv: After silencing ofβ-catenin protein by siRNA, whether the invasiveness of entopic endometrium which from patients who suffered from endometriosis will be changed. Explore the effect ofβ-catenin protein to change invasiveness of entopic endometrium.Methods: Endometrial stromal cells from eutopic endometrium of 24 women with endometrisis(diagnosed by pathology eventually) were divided into 3 subgroups at random: blank subgroup(n=8), negative subgroup(transfection by negative fluorescien control siRNA, n=8) and siRNA interference subgroup(transfection byβ-catenin siRNA, n=8).Observe the invasive characteristics of differental handling cells by in vitro invasion experiment using Transwell.Results: the number of invasion cells in endometriosis group(132.9±14.1)is higher than the normal group(5.5±2.4)(P﹤0.01). After transfection,β-catenin siRNA interference group(53.9±7.3) shows significantly less invasiveness than the negative control(91.1±4.5) and blank group (132.9±14.1)(P﹤0.01). and the last two groups have no obvious difference(P>0.05).Conclusion: The date suggests that siRNA ofβ-catenin can effectively restrain the invasion ability of entopic endometrial stormal cells of people who suffered endometriosis. This point out that Wnt/β-catenin signaling pathway may play a critical role in endometriosis.