Lipopolysaccharides On Expression Of Cxcr4 In Mouse Bone-marrow Smooth Muscle Progenitor Cell.

Aims:Through observing the effects of 1ipopolysaccharides on exsomatize mice spinal marrow non-odontogenic smooth progenitor muscle cell membrane surface stroma cells derived factor receptor expression, to discuss the role that SDF-1/CXCR4 axis plays in the developing process of atherosclerosis formation, thus to seek the possible ways of anti atherosclerosis.Methods:Using enhancement type P green-fluorescing cells plasmid recombinant to select the smooth progenitor muscle cell out from the mice marrow mesenchyma stem cells , then subculture them and divide them into six groups as the experiment's design:①Normal control group: add ordinary culture medium;②Low dosage group(group L): final concentration of the LPS is 1ug/mL, incubating for 6h;③Medium dosage group(group M):final concentration of the LPS is 10ug/mL, incubating for 6h;④High dosage group(group H): final concentration of the LPS is 30ug/mL, incubating for 6h;⑤group12h: final concentration of the LPS is 10ug/mL, incubating for 12h;⑥group24h: final concentration of the LPS is 10ug/mL, incubating for 24h. Use Western blotting and immunofluorescence staining and confocal laser scanning microscope to detect smooth progenitor muscle cell membrane surface stroma cells derived factor receptor protein expression and to use reverse transcription polymerase chain reaction to detect smooth progenitor muscle cell membrane surface stroma cells derived factor receptor mRNA's expression, thus to observe the relations between lipopolysaccharide's aging and concentration when the smooth progenitor muscle cell is expressing stroma cells derived factor receptor. Results:The immunofluorescence staining and confocal laser scanning microscope method have got the identical results with RT-PCR and Western blot detection, those smooth muscle progenitor cells that haven't been given lipopolysaccharide stimulation have stroma cells derived factor receptor expression of foundation level, lipopolysaccharide is a strong inducer of stroma cells derived factor receptor. When the stimulus time is invariable(6h), as the increasing of the stimulus concentration, stroma cells derived factor receptor expression of group L and group M strengthened gradually, and has significant difference with the normal control group(P<0.01), but group H began to decrease. When the stimulus concentration is invariable(10ug/mL), as the extend of the time, the expression volume of group M and group 12h increased as well, and has significant difference with normal control group(P<0.01). In all the groups, group 12h has the strongest expression, and it's mRNA and protein level are 6.10 times and 4.76 times as the foundation level respectively, the group 24h decreased, while still higher than the foundation level.Conclusion:Smooth muscle progenitor cells can express CXCR4 of low level, Atherosclerotic risk factors can lipopolysaccharide smooth muscle progenitor cells increases the level of CXCR4, the effect present concentration and time-dependent, SDF-1/CXCR4 axis involved in the smooth muscle progenitor cells vascular injury in the homing site.