Low-frequency Repetitive Transcranial Magnetic Stimulation On Atp Enzymes, Mda And Sod Of Central Nervous System In Immune-mediated Motor Neuron Disease Guinea Pig

Objective To study the effects of low-frequency(one hertz) repetitive Transcranial Magnetic Stimulation (rTMS) on ATPases, MDA and SOD of guinea pig with immune-mediated motor neuron disease in central nervous system. To provide a theoretical basis for the application of rTMS in the treatment of motor neuron disease(MND). Methods Models of immune-mediated motor neuron disease in guinea pig were established by using fresh bovine spinal cord anterior horn homogenate to immune guinea pigs and then divided into three groups:control group;pseudo treatment groups and treatment groups (The treatment group was given by rTMS at 1Hz five days a week). The differences of ATP enzymes, MDA and SOD were compared the treatment group, non-treatment group and control group by the method of Chemical assay, thiobarbituric acid and xanthine oxidase detected in cerebral cortex and spinal cord of guinea pigs after two weeks'treatment. Results Compared to the pseudo- treatment group, there was a clear statistically significant discrepancy in the treatment group:The activities of the total ATP enzyme and sub-type ATP enzyme of cerebral cortex and spinal cord rised(P=0.0192<0.05);The content of the MDA of cerebral cortex and spinal cord decreased (P=0.0175 <0.05);The activities of the total SOD and Cu/Zn-SOD of cerebral cortex and spinal cord rise. (P=0.0203<0.05). Conclusion Our data has been shown that the mitochondrial dysfunction and oxidative damage were possibly involved in the pathogenesis of the immune-mediated motor neuron injury. Low-frequency repetitive transcranial magnetic stimulation may play a therapeutic role to motor neuron disease by protecting mitochondrial function.