| Objective: To profile the expression of FOXC2 and explore its role in the advancement ofendometrial cancer.Methods: The expression of FOXC2 mRNA in both endometrial carcinoma cell lines ishikawa and hec-1A were determined by RT-PCR. After the stimulation with TGF-β1 in different concentration, FOXC2 mRNA was analyzed with RT-PCR. Immunohistochemistry was performed to detect the protein expressions of FOXC2 and its association with microvessel density (MVD )in 20 endometrial carcinoma tissues,10 complex hyperplasia endometrial with atypical hyperplasia and 5 normal control group tissues.Results: The expressions of FOXC2 were detected in both endometrial cancer cell lines ishikawa and hec-1A. TGF-β1 remarkably stimulated the expression of the FOXC2 in a dose-dependent manner. Moreover, FOXC2 protein could be detected in endometrial cancer cell membrane or cytoplast .The expressions of FOXC2 was higher than that in complex hyperplasia with atypical hyperplasia and normal control (P﹤0.01).FOXC2 expression level was positively correlated with MVD in endometrial carcinoma tissue samples(P﹤0.01).Conclusion: FOXC2 expression was related to angiogenesis in endometrial cancer and may play an important role in its invasion and progression. |
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