Extraction And Separation Of Alkaloids In Aconitum Herbs And Studies Of Esi-ms~n On Aconitine-type Alkalaids

As first recorded in 'Shen Nong's Herbal Classic', aconitum species have unique pharmacological activities and were collected by the Chinese pharmacopoeia. The major active component of aconitum species is C₁₉ type diterpenoid alkaloid. In this study, electrospray ionization mass spectrometry (ESI-MS) was utilized as the principal approach to investigate the extraction and separation process of alkaloids, the hydrolysis mechanism of di-ester type diterpenoid alkaloid, and the establishment of HPLC and MS Fingerprint of aconitum species with the aid of LC-MS.1. The aconitine type diterpenoid alkaloid was studied under the optimized condition after exploring the parameters of the ESI-MS. Such first order mass spectrum process was also useful to primarily understand the activities of alkaloid. The initial study on the mass spectral behavior of various di-ester type diterpenoid alkaloid and ester type alkaloid was completed by electrospray ionization mass spectrometry. As a result, according to the fragmentation manner of aconitine type diterpenoid alkaloid, the chemical structure related active locations of the di-ester type diterpenoid alkaloid were confirmed by using the second contacts mass spectrum.2. A preliminary research on the extraction of alkaloids from the aconitum species was also carried out. Under the optimized the extraction process and a suitable process route, the extraction ratio of total alkaloid is over 90 %. In addition, a series of approaches were explored here to separate 3 spices of alkaloid from the total alkaloid. The afterward electrospray ionization mass spectrometric assessment demonstrated that those methods were feasible for the separation of each 3 alkaloid.3. Based on the theory of organic chemistry, the hydrolysis mechanism of di-ester type diterpenoid alkaloid was emphatically discussed. Collectively, the broken of the C-O via a basic hydrolysis was confirmed and was introduced as the theoretical basis for the further herbal medicine processing and toxicity attenuation of aconitum species. Our conclusions were also supported by the ESI-MS assessment on the standard control sample of the di-ester type diterpenoid alkaloid.4. According to the effective parts from the diterpenoid alkaloids, the alkaloidal monomers for each monomer were extracted based on a certain alumina column chromatographic separation condition. This work provides the advanced qualitative and quantitative study on the aconitine type diterpenoid alkaloid with material guarantee5. The HPLC and MS fingerprint of aconitum species was established on the basis of an overall investigation on the changes in di-ester type diterpenoid alkaloid production, therefore, the differences of chemical constituents among the herbs can be comprehensive compared.