| Luidia:quinaria Von Martens,which belongs to class Asteroidea of phylum Echinodermata, constitutes about 24 percent of the total starfish resources along the Shandong coastal area.It is widely.distributed in the relatively higher salinity waters of Central Bohai Sea and the inshore waters of Yatai and Weihai fishing grounds.The aim of this thesis is to lay a solid foundation for aftertime development and utilization of such biological resources and provide a basis for development and utilization of Luidia Quinaria Von Martens though the study of Luidia Quinaria Von Martens' main active substances and the activity of its acidic mucopolysaccharide.(1) Extract Acidic Mucopolysaccaride from the Luidia quinaria von Martens with alkaline aqua.The contents of the polysaccharide and the protein of the gross product are 29.5%,65.7%, After the hydrolysis with enzyme,they are 63.2%,7.0%respectively.Also there is sulfate group in the product.One group of the products is further purified using ion-exchange chromatography.It is isolated through molecular sieve chromatography,and contains three components at least.Its average molecular is about 12kD.The result of agar gel electrophoresis showed that the product is Acidic Mucopolysaccharide.(2) The full oil of Luidia quinaria Von Martens is extracted and refined with the method of solvent extraction combined with silica gel column chromatography,of which the yields is 1.18%. The resuits of gas Chromatography analysis indicate that,the Luidia quinaria Von Martens contains abundant PUFA which is added up to 24 varieties,especially rich in EPA and DHA.The enrichment of EPA.and DHA can be achieved by taking advantage of silica gel column chromatography.After the column chromatography,the total PUFA content in ligarine is 57.63%, in which EPA and DHA take up 46.06%and 3.93%respectively;the total PUFA content in acetic ether is 58.15%,in which EPA and DHA take up 42.35%and 3.60%respectively.Thus it can be seen that,the Luidia quinaria Von Martens is a favorable source to extract PUFA.(3) The purpose of the trial is to extraction and separation asterosaponin.The total asterosaponin is prepared from Luidia quinaria von Martens with the methods of 85%ethanol repeated abstraction,petroleun ether defat,aturation normal butyl alcohol extraction and acetone sediment. The refined asterosapoininâ… ,â…¡,â…¢,â…£can be obtained respectively through Macroreticular resin and Sephadex LH20 chromatography,Checking with the HPLC,the refined asterosaponinâ… 1,â…¡2 can get single peek,and they are belong to steroidal saponins that can be testified by a series of the foam experiment,color reaction and precipitation reaction.(4) Activity assay of acidic mucopolysaccharide:The purpose of the assay is to study the Hypolipidemic effect of luidia quinaria von martens' GAG by taking Kumming mouse as the experimental animal.The results show that GAG can remarkably decrease the level of the high-fat diet in mouse's TG,TC and LDL-C and increase the level of the mouse's LDL.The GAG can also effectively control the rise of the high-fat diet in mouse's blood fat and keep all the blood fat indicators within the limits of normal scope.The external antitumour experiment based on the acidic mucopolysaccharide semifinished product by using MTT method shows that experimental group 1.0mg/ml has remarkable antitumor effects activity and its inhibition rate to HL-60 cells can reach 58.2%within 24 hours.To sum up,as a small classification of marine organism,Luidia Quinaria Von Martens has varous nutrients and many active substances which can be used as health food and medicine. |
PDF Full Text Request