The Research Of Improving And Application Of Fluorescence Molecular Imaging System

Fluorescence molecular imaging (FMI) is an emerging strategy in surgery and biological experiment and has remarkable advantages of convenience, simple, high sensitivity, multi target marked and dispense with substrate. With the injection of high-specificity fluorescence molecular probe in tissue, FMI offers noninvasive detection on the cell and molecular level, it can do the early diagnosis about caner and enhancing the rate of drug exploitation, FMI has very good foreground of development.In order to do FMI, using high sensitivity cool CCD as the low-light detector, based of a fiber-based tomography imaging system, develop a non-contact FMI system. This system is available to illuminate the specimen and obtain the fluorescence projection data from any angle. In order to investigate the FMI problem, designing some phantom and vivo experiments for improving the imaging system and enhancing the experiment methods. Tissue phantom experiments conducted on this system, the reconstructed image can reflect the position of the fluorescence target.Based on non-contact FMI system, develop the multi-band fluorescence molecular imaging system. This system can excite fluorescence probe and detect the emission signal on different excitation band and emission band. This system tide over the disadvantage of the former system which based on laser source, this advantage can make the system apply on practice situation.Present some in vitro and in vivo experiment on non-contact and multi-band fluorescence molecular imaging platforms, the experiment platforms have been proved which can research on in vivo optical imaging. Present the preliminary research of autofluorescence and digest process of mice by the in vivo experiment, which can act as the reference for the improvement of further experiment system platform.