Circulating Chemokine Cxcl16 Expression In Peripheral Blood Of Patients With Coronary Artery Disease

BackgroundCoronary disease is one of the commonly mortality diseases.Athero- sclerosis is characterized by a complex multifactorial pathophysiology.The mechanisms behind this phenomena have been unknown.Acute coronary syndrome(ACS) was the most emergency and servity,which also caused by the rupture of thrombosis,leading to unstable angina pectoris(UAP),acute myocardial infarction(AMI) and a sudden death.The unstable plaque play a important role in the progress of ACS.Vascular inflammation plays a major role in the initiation,progression and the final steps of atherosclerosis,namely,plaque destabilization and eventually plaque rupture. Chemokine was becoming the hot point for inducing inflammatory reaction. Chemokine is a small secreted protein molecular,which play a important role in the inflammatory reaction by recruited T Lymphocytes and Monocytes into decidua。CXCL16 is an indicited transmembrane molecule combining scavenger receptor functions with the properties of an inflammatory chemokine.As a transmembrane molecule,CXCL16 can act as a scavenger receptor by uptaking ox-LDL to form foam cell from macrophages,promoting the development of atherosclerosis.However, the role of the circulating soluble CXCL16 in human coronary heart disease(CHD) is still controversial.OBJECTIONThe aim of our study was,therefore,to investigate the role of CXCL16 in the development of atherosclerosis,finding a molecular marker for early diagnosis,risk evaluation and prediction for cardiovascular events. METHODS69 patients diagnosed with cronary artery disease(CHD) at our cardiovascular department,including 16 acute myocardial infarction(AMI) patients and 26 unstable angina pectoris(UAP) patients,and 27 patients with stable angina pectoris(SAP) were enrolled in this study.19 patients with non-coronary heart disease(CHD) served as controls.All cases received coronary arteriography.Serum-soluble CXCL16, TNF-a and IL-8 was obtained by commercial ELISA and Gensini score was calculated by Judkins.Other parameters including HDL-C,LDL-C,TG,TC,WBC,Cr was detected by general biomedical method.The family history of DM,blood hyperpressure and CHD was collected.RESULTS1.The level of CXCL16 in peripheral blood in UAP and AMI groups were significantly higher than those in SAP group and normal controls(P＜0.05);however, there was no significant difference in CXCL16 expression between UAP group and AMI group(P＞0.05),nor did between SAP group and normal controls.2.The serun level of TNF-a and IL-8 in SAP,UAP and AMI groups were significantly higher than those in normal controls(all P＜0.01),the UAP group is significantly higher than SAP group(P＜0.01),and the AMI group is the significantly higher than UAP group(P＜0.05).3.Gensini score was significantly increased in UAP and AMI groups compared to SAP group(P＜0.01),But no significant difference is found between UAP and AMI groups(P＞0.05).4.No significant association was found between serum CXCL16 and Gensini score,nor did between TNF-a,IL-8 in control and SAP group,however,significant correlation was found between CXCL16 and TNF-a,IL-8.There is significant correlation between TNF-a and IL-8 in serum.(r=0.711,P=0.000)5.There was no statistically significant difference(P＞0.05) in sex,weight, disease history,family history,taking medicine.The number of the WBC in AMI groups were significantly higher than those in SAP group(P＜0.05) CONCLUSIONSCXCL16 may play an important role in the pathogenesis and development of acute coronary syndrome(ACS) in humans,starting up the coinefficiently effect between TNF-αand IL-8 as a primer regulating factor.