| Human bone is a bioceramic composite that is mainly composed of collagen and hydroxyapatite (HA), in which both high strength and fracture toughness can be achieved due to the unique architecture of this organic-inorganic composite. Titanium (Ti) and its alloys are necessary in clinic due to their significant application in the biomedical areas such as loaded artificial bones. But their fixation in the body is unsolved problem because of their bioinert surface. Plenty of techniques have been developed to modify their surface character in order to realize their biological bond to natural bone.In the present study, a biomimetic process was employed to form a hydroxyapatite (HA) coating on alkali-heat treated titanium (Ti) substrate in the 1.5 times simulated body fluid (1.5×SBF) with the addition of a recombinant collagen-like protein. The coating was characterized using SEM-EDS, FESEM, XRD and FTIR. Results showed that the recombinant collagen-like protein could accelerate the preferential nucleation and directional growth along c axis of HA on the pretreated Ti substrate. The modulation mechanism of the collagen-like protein on HA crystals was discussed. The effect of coating composed of HA and protein composite on MG-63 cells was investigated in vitro. The existence of protein in coating could improve the initial cell adhesion, proliferation and differentiation of MG-63 cells, which implied the materials possessed excellent biocompatibility and had a wide potential in biomedical application.This work may provide an interesting idea in the design and fabrication of bioactive coating on the surface of biomedical metal for the clinical application. |
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