Biocompatibility And Antibacterial Properties Of Pure Titanium Surfaces Coated With Yttrium-doped Hydroxyapatite

Background: The repair and regeneration of peri-implant soft tissues is essential for the long-term and short-term clinical successes of implants.Surface modification of implants using coatings is an effective approach to improving their biocompatibility and antibacterial properties.objectives: In this study,we introduced a novel implant material by modifying the surface of pure titanium(Ti).Hydroxyapatite(HA)and HA doped with different concentrations of yttrium(Y)were coated on the surface of pure Ti substrate via sol-gel process.Methods: Hydroxyapatite coatings and different concentrations of cerium-containing hydroxyapatite coatings were successfully prepared on the surface of pure titanium by sol-gel method.The surface of the sample was characterized by scanning electron microscopy,atomic force microscopy,contact angle measuring instrument,X-ray electron spectrometer and X-ray diffractometer.Adhesion,proliferation and morphological spreading of human gingival fibroblasts by different concentrations of hydroxyapatite coating on pure titanium substrate by CCK-8 cell proliferation assay,AO/EB fluorescence staining,scanning electron microscopy and atomic force microscopy The effect of collagen synthesis.antibacterial properties against Streptococcus mutans.Results: Surface characterization indicated that the coatings did not significantly increase surface roughness.The modified coatings significantly enhanced the adhesive,proliferative,and spreading capacities of HGFs compared to the pure Ti substrate.Col-1 secretion by HGFs positively increased with increased Y doping and duration of cell cultivation,suggesting that the coatings may promote connective tissue formation.Furthermore,increased Y doping significantly reduced the number of adherent S.mutans.Thus,Y-doped HA coatings improve biocompatibility and antibacterial properties,suggesting they have high potential for improving the repair,regeneration,and integration of soft tissues on the surfaces of Ti implants.