Hydrogen Sulfide In Renal Ischemia Reperfusion Injury And Its Mechanism

Objective To observe the role of oxidative stress of the different doses of hydrogen sulfide on ischemia reperfusion injury (IRI) renal, and to explore the role of renal ischemia reperfusion injury and its mechanism.Methods 32 healthy male Wistar rats were randomly divided into 4 groups, namely sham operation (sham operation, Sham) group, renal ischemia/reperfusion(renal ischemia/reperfusion,I/R) group, sodium hydrosulfide (NaHS) high dose group, Sodium hydrosulfide low dose group, n=8.After excision of the right kidney, sodium hydrosulfide (NaHS) as a donor of hydrogen sulphide of animals, left renal artery of two dose NaHS group were given 300nmol/min,1.5umol/min 15min continuous administration, Sham group and I/R group Given the same volume of saline, stop drug for 5min, NaHS group and the IR group folders Clipped the left renal pedicle with no damage arteriole occlusion, after 45 minutes blockade, so established a model of acute ischemia reperfusion injury renal, the sham operation group Without clamping the left renal artery, the other operation is the same model group.Reserved renal tissue specimens and blood in 24 hours after restore blood flow in the kidney, serum urea nitrogen (BUN), creatinine (Scr); kidney tissue superoxide dismutase (SOD), malondialdehyde (MDA), semi-quantitative analysis of kidney Pathological damage, and in situ end labeling (TUNEL) detection of renal cell apoptosis.Results The kidneys of sham operation group were normal, operated rats appeared acute tubular necrosis, the most importantly pathological damaged was IRI group, sodium hydrosulfide (NaHS) pretreatment significantly reduced pathological damage;SOD activity was significantly lower in IRI group, MDA content increased significantly, a large number of renal tubular epithelial cells;SOD activity was significantly increased in Sodium hydrosulfide group, MDA formation was significantly reduced, Reduced the renal tubular epithelial cell apoptosis;There were no difference between two NaHS doses groups.Conclusion Hydrogen sulfide on ischemia-reperfusion injury renal has a protective effect, Part of the mechanism may be related to scavenging oxygen free radicals, lipid peroxidation, reducing cell apoptosis.