| Objective:1.To explore the anti-tumor and anti-cisplatin side effects of Pu-erh combined with Ginsenoside,and their joint application for inspection of biological safety.2.Compare Prevention research and Treatment research results of Pu-erh combined with Ginsenoside anti-tumor and anti-cisplatin side effects study.Methods:1. Pu-erh combined with ginsenoside of anti-tumor and anti-cisplatin chemotherapy side effects prevention research:first,rats were given free diet, drinking tea, ginseng saponin fed for two months. And then inoculated tumors, observed and recorded the rate of tumor and tumor volume. After the tumor volume of tumor reached 1cm3,the intraperitoneal injection of cisplatin, and serum collected 0,3,7,15,30 days, testing ALT, AST, BUN, CR liver and kidney function,recorded once every three days.30 days later,kill all rats and collect the liver, kidney,tumor tissues. Another frozen liver and kidney tissue remaining measured antioxidant SOD, CAT, GSH-PX, MDA and NO.2. Pu-erh combined with ginsenoside anti-tumor and anti-ginsenoside side effects of chemotherapy with cisplatin treatment, therapy:the first tumor inoculation, observation and record the rate of tumor composed of tumor volume. After the tumor volume of tumor reached 1cm3, the intraperitoneal injection of cisplatin. Rats were given free diet,drinking Puer tea, ginseng saponin fed, the serum collected 0,3,7,15,30 days, testing ALT, AST, BUN, CR liver and kidney function, recorded once every three days.After 30 days, all rats were killed and collect liver, kidney and tumor tissues. Another frozen iver and kidney tissue remaining measured antioxidant SOD, CAT, GSH-PX, MDA and NO.3. In vitro Pu'er anti-tumor research:Tfour concentrations (0.15mg/ml, 0.25mg/ml,0.35mg/ml,0.45mg/ml) with the tea culture of human breast cancer MCF-7 were cultured 12h,24h,36h,48h, usingMTT and CCK-8 was detected by MCF-7 human breast cancer cell proliferation inhibition.4. In vitro anti-inflammatory of tea:RAW264.7 macrophages were cultured in, take the best point of state boards. With 0.45mg/ml tea culture medium with concentrations were incubated 12h,48h. Supernatant was collected. ELISA assay of cytokines by IL-1β, IL-6, TNF-α. LPS as a positive control in each group.Results:1. Pu'er Tea combined with ginsenoside prevention is better than treatment in anti-tumor area.Prevention of the experiment, the control group tumor volume increased faster; other slow growth of tumor volume in experimental group, and tumor volume was significantly lower than cisplatin group, indicating that the inhibition of tumor growth in experimental groups are the effect; therapy experiments,tumor volume growth in all experimental groups.Faster and significantly greater than CDDP group.Prevention of the experiment,15-18 days after administration of cisplatin, because cisplatin nephrotoxicity caused an increase in mortality in each group; treatment experiment,5-10 days after administration of cisplatin, an increase in mortality in each experimental group.We can know Pu'er and ginseng saponins can effectively prevent cisplatin-induced toxicity, thus prolong survival.2. Pu'er Tea combined with ginsenoside in the prevention of side effects of cisplatin chemotherapy is better than treatment.Prevention experiments, saponin group and Pu'er Tea combined with ginsenoside can efficient inhibit the increase of BUN and has a significant difference. Pu'er tea is also effective but not significant. Pu'er group, ginsenoside groups and Pu'er Tea combined with ginsenoside showed strong inhibition the increased of CR, and the three groups showed no difference. Treatment experiment, the BUN and CR values of Pu'er group,saponin group, Pu'er Tea combined with ginsenoside and CDDPgroup were significantly higher compared with the control group, and each group showed no significant difference. Instructions, pu'er tea, ginseng saponins and Pu'er Tea combined with ginsenoside can be used to guide the prevention of cisplatin induced renal injury, but as atherapeutic drug has no effect.Prevention experiments, ALT values compared with the control group, other groups were not significantly different. Description7mg/ml dose cisplatin toxicity of cisplatin did not cause liver damage.3. Pu'er tea on human breast cancer MCF-7 inhibited the proliferation of time and concentration into the dose-dependent. With a total incubation time, the tea concentration, inhibit the proliferation of MCF-7 cells to streng then.4. Renal tissue antioxidant capacity:1. Cisplatin on the prevention of renal tissue SOD activity has little effect and significantly increased the accumulation of MDA and NO and significantly reduced GSH-PX and CAT activity.2.Pu'er Tea may be mainly through the CAT activity was significantly enhanced and more effective than ginseng saponin, To achieve the enhancement of tissue antioxidant, effective in preventing cisplatin-induced oxidative damage. Pu'er tea can also improve the antioxidant capacity by SOD and GSH-PX activity and reduce cisplatin-induced accumulation of MDA and NO, but the effect was not significant.3. Ginsenosides on cisplatin-induced oxidative damage have some preventive effect. Can enhance the SOD and GSH-PX activity, but the role of CAT activity did not increase; decreased tissue accumulation of MDA, the effect is inferior to Pu'er ginseng combination group, but better than the pu'er tea; It can significantly reduce the tissue accumulation of NO, better than the pu'er tea. 4. Pu'er Tea combined with ginsenoside, showing the superior effect of tea and ginseng saponin. Showed strong enhancement of tissue antioxidant capacity.It can significantly enhance CAT activity, The effect was significantly lower than the pu'er tea, but better than ginseng saponin;, NO significantly decreased the accumulation, the effect is better than pu'er tea; enhanced tissue SOD and GSH-PX activity, decreased tissue MDA accumulation, The effect is better than pu'er tea and ginseng saponins, but no significant difference.5. Prevention of hepatic antioxidant capacity:1. cisplatin can reduce the tissue antioxidant capacity, causing lipidperoxidation. mainly through significantly reduced tissue SOD, GSH-PX activity, significantly increased tissue MDA content,reduced CAT activity increase the NO content.2.Pu'er tea have some preventive effect on oxidative damage by cisplatin. Significantly increased SOD activity, but the effect is inferior to Pu'er tea combined with ginsenoside. CATactivity increased significantly, and the effect is superior Pu'er tea combined with ginsenoside and ginsenoside. GSH-PX activity increased, decreased tissue NO content, but no significant difference.3. Ginsenosides significantly increased GSH-PX activity and reduce the accumulation of tissue NO, and better than Pu'er tea. Increase CAT activity, but no significant difference. Ginsenosides have demonstrated effective prevention of tissue peroxidation.4. Pu'er tea combined with ginsenoside,the activity of anti-peroxidation is great higher than Pu'er and Ginsenosides. The significantly enhanced SOD, GSH-PX, CAT activity significantly decreased the accumulation of NO.Conclusion:1. The Pu-erh combined with ginsenosides in the anti-tumor and anti-cisplatin effect of prevention is better than treatment. |
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