Liquorice Before And After The Algae Compatibility Changes In The Chemical Composition And Fingerprint Study

The optimal extraction process of Glycyrrhiza uralensis was studied by orthogonal design with the contents of glycyrrhiza acid , four factors were investigated in this experiment, including the methanol concentration, solid-liquid ratio, time of extraction and times of extraction. The results showed that the optimal extracting condition was 60% methanol, solid-liquid ratio 1:12, refluxing for third and 1 hour each time at 60℃. RP-HPLC methods were established to determine glycyrrhiza acid in the herb couples. The HPLC separation was achieved on a Kromasil-C18 (250 mm×4.6 mm, 5μm) column at 30℃. The mobile phase consisted of methanol-acetonitrile-0.2 mol·L^-1 ammounium acetate-glacial acetic acid (45:15:40:1), the UV detection wavelength was 250 nm, flow velocity was 1.0 mL·min^-1. The linear range was 39.18¹95.90μg·mL^-1(r = 0.9999). The average recoveries ( n =9 ) of the glycyrrhiza acid was(100.2±1.5)% . The unitary content diversity of the ration 1∶1 in Glycyrrhiza uralensis after combining with Sargassum fusiforme at different rations was the greatest via the data. The method is rapid , accurate , credible and repeatable , and can provide bases for the efficiency increase and toxicity reduction in Glycyrrhiza uralensis compatible with Sargassum fusiforme.The optimal extraction process of Sargassum fusiforme was studied by orthogonal design with the contents of sargassum fusiforme polyccharides, three factors were investigated in this experiment, including the solid-liquid ratio, temperature of extraction and time of extraction. The results showed that the optimal extracting condition was PH=3.0 acid water, solid-liquid ratio 1:30, refluxing for twice and 5 hour each time at 85℃. H₂SO₄一minophenol method were established to determine sargassum fusiforme polyccharides in the herb couples. The linear range was 20¹00μg·mL^-1(r = 0.9999). The average recoveries ( n =9 ) of the sargassum fusiforme polyccharides was(100.3±0.7)% .With Glycyrrhiza uralensis compatibility of increasing the proportion of the contents of glycyrrhiza uralensis polysaccharide gradually enhance, however, compatibility of total polysaccharides quantity reduce gradually, this elucidation compatibility, affect effective ingredients of sargassum fusiforme polysaccharides changes, may is due to the change in the composition of the cause of curative effect is reduced, perhaps can reveal compatibility taboo material base.HPLC fingerprint spectrumof Radix Glycyrrhizae was established in order to provide reference for its quality control . RP-HPLC was used in the determination of Radix Glycyrrhizae by gradient elution under the following conditions : column of Kromasil-C18 (250 mm×4.6 mm, 5μm), flow rate of 1.0 mL·min^-1 , detection wavelength of 235 nm and acetonitrile (A)- 0.08% phosphoric acid water (B) as mobile phase. 10 batches of Radix Giycyrrhizae were determined and 21 distinguished peaks were found in the HPLC fingerprints spectra. Thismethod is accurate and steady with a good repeatability and p roviding a reference for quality control of Glyrrhiza uralensis.