Traditional Chinese Medicine To Eradicate Helicobacter Pylori (in Vitro Resistance Response Experiment)

Purpose:This study chooses recognized fight helicobacter pylori effect good astragalus, sophora flavescens, dandelion, rhizoma coptidis, born licorice five medicine as the research object, by different doses of these five drugs square composed of three groups optimization Hp active resistance was studied, through experimental study induced resistance reaction for traditional Chinese medicine, and the resistance of h. pylori eradication provides a new theory basis for traditional Chinese medicine, also broadening the range.Materials and methods: preparation of medium and petri dishes, activate and appraisal h. pylori, preparation bacterium fluid, experimental group is divided into group (Chinese medicine induction group, metronidazole induction group, clarithromycin induction group), blank control, solvent control group. Using many steps on the YouDaoFa resistance test in vitro HP chose clinical resistance high metronidazole and currently resistance to HP curative effect good clarithromycin synchronized trial; Observe the drug concentration gradually increase in medium of HP minimum bacteriostasis have instead been growing concentration (MIC) of change; Will induce the drug-resistant strains of continuous have instead been growing 10 times determination to determine after drug resistance, MIC whether stability; Will decline in the sensitivity no medicine medium induced strains have instead been growing three times after testing on M1C value, determination of optimal formula three groups of drop induction strains sensitivity metronidazole M1C values.Results:1.The multi-step induced in the process of cultivation, to streamline party not induce, reduced two sides and streamline tripartite drug-resistant strains of HP, while in contain 64 times MIC metronidazole medium strains can still grow, on 4 times MIC clarithromycin in the strains can still grow medium. 2.In the process of cultivation, the sensitivity of metronidazole strains gradually declined, M1C value increasing gradually to 64ug/mL; Metronidazole drug-resistant strains (MR) medium without medicine have instead been growing 10 times later got MR 'the MIC values; Strains of sensitivity also to clarithromycin gradually declined, M1C value increasing gradually to 4ug/mL; Clarithromycin drug-resistant strains (CR) medium without medicine have instead been growing 10 times later got CR 'the MIC values.3.Streamlining party, the simplification of two sides and streamline the tripartite to MR and CR no impact value MIC.Conclusion:1.This study found that HP on three groups of optimization prescription drug resistance reaction, not easy to generate for metronidazole and clarithromycin HP to produce resistance reaction.2.HP to metronidazole and clarithromycin produce resistance to relatively stable .3.Metronidazole and clarithromycin resistant strains on three groups of optimizing formula remain sensitivity.