Ki-67 Antigen And Bcl-2 Protein Expression In Lymphoproliferative Disorders

Objective To evaluate the expression of proliferative antigen Ki?7 and apoptosisa antagonizing Bcl? and theirs clinical significance in nonodgkin's lymphoma (NHL) and acute lymphoblastic leukemia (ALL) Methods The Ki?7 antigen and Bcl? protein were measured by immunohistochemistry on formalinixed, paraffin mbedded tissue sections in 35 cases of NHL and bone marrow or peripheral blood samples in 120 cases of leukemia. Results 1. Ki?7 antigen was recovered by the antigen retrieval technique of heating, trypsin digestion and heating in a microwase oven on formalinixed, paraffin mbedded tissue sections. The way of staining pattern of heating in a microwase oven for Ki?7 antigen retrieval is the best method. 2. The expression of proliferative antigen Ki?7 and apoptosisantagonizing Bcl? in NHL and theirs clinical significance. (1) The level of Ki?7 expression in low grade NHL was lower than that in high grade NHL,and Bcl? expression in lower grade NHL was higher than that in high grade. (2) The level of Bcl? expression in B cell NHLwas higher than 5 that in T cell NHL. There was no significant difference in the expression of Ki?7. (3) The level of BcF2 expression in remission group was lower than that in nonemission group during the first chemotherapeutics. There was no significant difference in the Ki?7 expression. (4) The survival time was longer in the group of Ki?720% than that in the group of Ki?7 >20%. There was no significant difference between high and low group in the expression of Bcl?. 3. The expression of proliferative antigen Ki?7 and apoptosisantagonizing Bcl? in adult ALL and theirs clinical significance. (1 )The level of Ki?7 expression in adult ALL was higher than that in acute nonymphoblastic leukemia(ANLL) and chronic lyrnphocytic leukemia(CLL). Bcl? expression in adult ALL was lower than that in ANLL and CLL. (2) The level of Ki?7 and Bcl? expression in TLL and My ALL were higher than that in BLL and nullLL, but there were no significant differences (3) The highest complete remission (CR) rate was in the group of both indexes (Ki?7 and Bcl?) lower expressed. The lowest CR rate was in the group of both indexes higher expressed. However, in the group that one of two indexes expressed higher, but the other expressed lower, the CR rate was in the middle. (4) The survival time was longer in the group of Ki?7 15% than that in the group of Ki?7 >15%. There was no significant difference between high and low group in the expression of Bcl?. Conclusions The level of Ki?7 and Bcl? expression were closely related with 6 the malignant grade and the prognosis of NHL and with the type of leukemia, chemotherapeutic effects and the prognosis of adult ALL.