Hirudin Experimental Traumatic Pvr

OBJECTIVE:To study the mechanism of hirudin on preventing and curing proliferative vitreoretinopathy (PVR),by observing the pathological changes of fundus oculi and eye with ophthalmoscope(Oph) and light microscope respectively of the experimental traumatic PVR model rabbit and measuring the changes of platelet derived growth factor (PDGF) in corpus vitreum and flash electroretinogram (F-ERG) of the model rabbit eye when PVR model rabbit is treated with injection of hirudin into the eye ball.METHODS:34 Japanese white rabbits were randomly divided into 5 groups:normal control group,model control group normal saline group,high and low dosage treatment group. All the groups were divided into short-term and long-term group respectively. Perforates eye injury combined injecting blood into vitreous cavity was adapted to establish PVR models. When the models were being made,high and low dosage treatment group of hirudin was injected into vitreous cavity separately. The fundus and routine histopathology were observed,ELISA was adapted to evaluate the density of platelet derived growth factor (PDGF) and the b-waves of flash electroretinogram (F-ERG) were recorded.RESULTS:(1) The fundus and pathological slides showed that the hyperplasia memberane of the high and low dosage groups of hirudin was less than that of the model group;(2) The density of PDGF hi vitreous cavity of high and low dosage treatment groups of hirudin was markedly lower than that of model group (P<0.05);(3) Both high and low dosage groups could elevate the amplitudes of F-ERG b-waves,which had significant difference (P<0.01) compared with the model group. The amplitudes of high dosage treatment group was higher than that of low dosage treatment group (P<0.05).CONCLUSIONS:Hirudin could prevent PVR from being formed or developing and had protective function on retina,whose mechanism related to the decrease of density of PDGF in vitreous body.