| As an important growth factor for improving the growth of the bone, Insulin-Like Growth Factor (IGF-I) is produced by Osteoblast through self-excretion or side-excretion. In order to find out the changing rule of IGF-I content inside the bone in the process of Osteoporsis induced by the loss of nerve control, and further investigate the pathogenesis and prevention measures of Osteoporsis caused by this reason, we established a model of Osteoporsis induced by the loss of nerve control. Through the dynamic observation on the IGF-I content of rats which lost the nerve control, we found out the changing rule; by examining AKP and TRAP in the bone, we revealed the function and mechanism of IGF-I in the local bone metastasis; by the comparison between the groups, we preliminarily explored the different functions that the loss of nerve control and the fixation perform in leading to Osteoporsis; through investigation of the content change of IGF-I after the passive movement and electrostimulation, we understood the function of Osteoporsis. Therefore this study will provide theoretical basis for the clinical prevention of Osteoporsis by IGF-I. The methodology:We took as samples four series of healthy adult male rats aged four weeks at clean level SD which totaled 144 rats and weighed 220g to 250g for each. After the adaptation for one week, the rats are divided into six groups: 1. the operation group with the loss of nerve control: to expose the ischiocele and the femoral nerve after anaesthesia and then excise 0.5cm to 1 cm before suture the part, and to cure the cut with antibiotics; 2. the comparison group: to expose the nerve under a pseudo-operation; 3. die fixation group: to fix the post legs with plaster, 4. the fixation group with the loss of nerve control: to make a fixation after cutting off the nerve; 5. the passive movement group with the loss of nerve control: to bend and extend the post legs after the loss of nerve control, twice a day and 20min/time; 6. the electrostimulation group with the loss of nerve control: to stimulate the post legswith 35Hz square wave after the loss of nerve control, twice a day and 20min/time.We killedthe rat by decollation, and took off 1/3 from the tibia and weighed. And then we put the part into uniform serum and uniformed it for 3 minutes, and then centrifugated it, and finally kept the upper clear liquid at 2 to 8 癈 for future examination. IGF-I examination: indirect ELISA mensuration (number DSL-10-2900, a product made by Diagnostic Systems Laboratories, USA). AKP and TRAP examination: velocity mensuration (a product made by Nanjing Jiancheng Bioengineering Research Institute). All of the data have been processed by SPSS software package with t-check for the comparison between groups and p<0.05 as the standard for markedness. The result:The IGF-I examination of each group of the samples demonstrates: (1) by comparisons inside the comparison groups, under the normal condition of the rats after Id, l0d, 30d, and 60d, there is no marked change in the IGF-I content of the bone; (2) by the comparison between the group with the loss of nerve control and the comparison group, the former group is obviously higher than the latter after 10d (p<0.05); (3) when compared to the comparison group, the fixation group is obviously higher than the comparison group after l0d (p<0.05) and obviously lower than the comparison group after 60d (p<0.05); (4) making a comparison with the comparison group, the fixation group with the loss of nerve control is obviously higher than the comparison group after l0d (p<0.01) and obviously lower than the comparison group after 60d (p<0.01); (5) compared to the fixation group with the loss of nerve control, IGF-I the content of the group with the loss of nerve control has markedly increased (p<0.05) after 60d; ?by comparisons with the comparison group and the group with the loss of nerve control, the electrostimulation group has its IGF-I level markedly increased (p<0.05).The bone AKP and TRAP activity examination for every group of sampl... |
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