Preliminary Analysis Of The Mouse Spermatogenesis-related Proteins

Spermatogenesis, the fundamental function of adult testis, is a continum of cellular differentiation in which three principal phases can be discerned: spermatogonia renewal and proliferation, meiosis and spermiogenesis. It is a complex process involving cell division, diffentiation and interactions between cells in the microenviroment of the seminiferous tubule. This differentiation process accompanies morphological changes that are controlled by a number of proteins expressed in a stage-specific manner during Spermatogenesis.The separating and identifying of proteins related to Spermatogenesis and the study of their molecular regulating mechanism at proteome level are very important for clinical diganosis and treatment of male infertility. It is also important to male molecular contraception.The rapidly emerging field of proteomics has now established itself as a credible approach for furthering our understanding of the biology of whole organism梖rom simple unicellular organisms to those as complex as man. The readily available experiment tools for measurement of protein expression by two-dimensional gel electrophoresis (2-DE), and for protein identification and characterization by mass spectrometry-based methods, have already made a significant impact on proteomics. The growing interest in the field looks set to accelerate the development and implementation of improved strategies for the analysis of proteinexpression and function on a genome-wide scale.Sophisticated bioinformatics tools are being developed to handle the vast quantity of data resulting from genomic and proteomics studies. Collections of proteomics databases for organisms such as yeast, contain biological information on proteins that assists with annotating and interpreting experimental results from proteomics studies.In order tcTisolate and identify proteins involved in spermatogenesis, we initially constructed technique platform for proteome analysis. Then proteome from 1 -week mouse testes and 7-week mouse testes were seperated and compared by 2-D electrophoresis. 49 protein spots highly expressed in 7-week mouse testis were excised and then digested in-gel by enzyme and the generated peptides were measured by MALDI-TOF mass spectrometry. 22 proteins were biochemically identified and characterized.In summary, we have constructed proteome analysis platform, by which 22 proteins were identified as related to spermatogenesis. In the proteomic era, all above works will become the fundamental theory basis of the functional research for spermatogenesis-related proteins, the drug screen and mechanism analysis, and the development of testis-associated clinic diagnosis and therapy of male infertility.