O-dibutyl Phthalate Exposure On The Male Reproductive System During Pregnancy

PratⅠEffects of maternal exposure to di(nbutyl)phthalate onprotein map of testes in embryonic male ratObjective: To isolate and identify differential expression proteins inembryonic rat testis after maternal exposure to di(nbutyl)phthalate(DBP).Methods: Ten pregnant rats were randomly divided into two groups and weredaily treated by gavage with 800mg/kg DBP (Group A) or 5mlcorn oil (Group B) from the 14th day to the 18th day of pregnancy.The embryonic testes were taken out at the 19th day of pregnancy,and total testis proteins were extracted. The proteins wereseparated by two-dimensional gel electrophoresis and identified bymass spectrometry.Results: Thirty-three differentially expressed protein spots (t≥2.831, p＜0.05)have been found and fourteen of which were identified by massspectrometry and searched in the SwissProt database. Thesedifferentially expressed proteins, such as Peroxiredoxin 6, AnnexinV and Ubiquitin carboxyl-terminal hydrolase isozyme L1, arerelated to apoptosis, cell proliferation and differentiation, signaltransduction, etc.Conclusion: Proteomics provides an effective method for studying the developmental toxicity of DBP to male reproductivedevelopment. Some of these differentially expressed proteinsmay provide evidence for us to further investigate the molecularmechanism of reproductive toxicity following DBP exposure.PartⅡDifferential proteomic analysis of hypospadiae male ratoffspring following maternal exposure to di(nbutyl)phthalateObjective: To isolate and identify differential expression proteins in ratoffspring genital tubercle after maternal exposure todi(nbutyl)phthalate (DBP).Methods: Ten pregnant rats were randomly divided into two groups and weredaily treated by gavage with 800mg/kg DBP (Group A) or 5mlcorn oil (Group B) from the 14th day to the 18th day of pregnancy.The genital tubercle of normal and hypospadiac male rat offspringwere respectively taken out on postnatal day (PND) 3, and totalgenital tubercle proteins were extracted. The proteins wereseparated by two-dimensional gel electrophoresis and identified bymass spectrometry. Results: Thirty-one differentially expressed protein spots (t≥2.831, p＜0.05)have been found and 17 of which were identified by massspectrometry and searched in the SwissProt database. Thesedifferentially expressed proteins, such as Pyruvate kinase M2,Alpha-enolase, Annexin A3, are related to histologicaldifferentiation, growth control, etc.Conclusion: Proteomics provides an effective method for studying thedevelopmental toxicity of DBP to male reproductivedevelopment. Some of these differentially expressed proteinsmay provide evidence for us to further investigate the molecularmechanism of hypospadias induced by DBP.