Expression Of Pten Gene In Primary Hepatocellular Carcinoma

Objective To investigate the function of PTEN alternations in the pathogenesis of hepatocellular carcinoma(HCC) and elucidate the molecular mechanism of it. Methods Semi-quantitive reverse transcriptase-polymerase chain reaction (RT-PCR) was adopted to detect the expression variation of PTEN gene in 30 HCC tissues.their corresponding adjacent liver tissues and in normal liver tissues of 16 non-liver disease patients. Results (1)26(86.7%) of the 30 HCC tissues expressed PTEN RNA while its positive expression rate was 100% in the adjacent and normal liver tissues.There were no significant difference in the expression rate of PTEN gene among the three kind of tissues (P>0.05): (2)The expression quantity of PTEN gene in the HCC tissues.its adjacent tissues and normal liver tissues wasl.619 0.407( x±s), 1.302 0.343(x=s) and 1.232=0.386( x±s).respectively. The expression quantity of PTEN gene in the HCC tissues was significantly lower than that in the adjacent and normal liver tissues(p<0.01); (3) The expression quantity of PTEN gene in the low-differentiated HCC tissue was also much lower than that in the high and middle differentiated HCC tissues(P<0.01); (4)The expression quantity of PTEN gene in HCC tissues with satellite focuses was not significantly different from that without satellite focuses(P>0.05): (5)The expression quantity of PTEN gene in HCC tissues was not closely related to HCC patients' age and genre (P>0.05). Conclusions (1)The negative expression rate of PTEN in the HCC tissues is low: (2)The expression quantity of PTEN in the HCC tissue is much lower than that in the adjacent and normal liver tissues, which indicates that the inactivation of PTEN is closely associated with the pathogenesis of HCC; (3)The expression quantity of PTEN gene is highly associated with the pathological differentiation of HCC,which indicates that the alternation of PTEN can serve as a prognostic indicators in the progression of HCC; (4) The expression quantity of PTEN gene in HCC tissues was not closely related to HCC patients' age , gender and whether there are satellite focuses in HCC tissues.