Medicine Manshuriensis The Experimental Study Of Acute Renal Toxicity

Caulis Aristolochiae Manshuriensis(CAM) is a common Chinese herb which has function of clearing away Heart-Fire, diuresis, promoting lactation. Its nephrotoxicity has recently got more and more attention in China and abroad. In our study, by means of advanced modern scientific research methods, we determined the content of total aristolochic acid in the stem of the CAM, observed the functional and pathological changes of acute renal failure of rats induced by CAM, investigated the effect of its nephrotoxicity making up Rheum palmatem L, Ligusticum chuanxiong Hort, Ligustrazine, Sodium Feralate, and firstly pilot studied the toxicokinetics of CAM. The result of experiments showed as below:1. The total aristolochic acid contents of CAM was 15.0mg/g, and total aristolochic acid contents of its decoction was 4.5mg/ml. Acute renal failure was induced afer SD rats were teated with 60g/kg d CAM decoction for 3 days, whose dose equivalent to that defined in 2.4066g/kg of total aristolochic acid contents.2. The acute renal failure of CAM (60g/kg d ) were lightened when making up Rheum palmatem L (2g/kg d) , Ligusticum chuanxiong Hort (2g/kg d) , Ligustrazine (8mg/kg d), which accord with the principle of mutual detoxication and mutual restrain between two herbs in Chinese traditional medicine Make-up theory.3. The total toxicants of CAM decoction were eliminated as first-order kinetics, and its distribution appeared generally to be a one-compartment model by our method. The apparent half-life tune of CAM decoction in mice was estimated to be 31.87h, which means that the elimination rate in the body is slow. We must control its interval time of using in clinic.