| ObjectiveBy setting up the myocardial ischemia-reperfusion injury model of rat andproducing relative experimental evidences, we would probe the mechanism andtheory of JinXiangDan protecting myocardial ischemia-reperfusion injury, and provide an experimental basis for further investigating and clinical use.Method1 110 healthy male SD rats, ranging from 180g to 220g, were divided into five groups at random: group Ⅰ (pseudo operation ); group Ⅱ (ischema-reperfusion operation); group Ⅲ (ischemia-reperfusion with JinXiangDan 1620mg/kg.d); group Ⅳ (ischemia-reperfusion with JinXiangDan 540mg/kg.d); group Ⅴ (ischemia-reperfusion with DiAoXinXueKang 54mg/kg.d); There were 22 rats in each group.2 The rats in group Ⅰ and Ⅱ were fed with 0.9% saline, while the rats in group Ⅲ,Ⅳ,Ⅴ were fed with 2.7mg/ml DiAoXinXueKang Liquor, 81mg/ml JinXiangDan Liquor, 27 mg/ml JinXiangDan Liquor respectively. Each rat was fed with 20ml/kg one time a day for ten consecutive days.3 The model of myocardial ischemia-reperfusion injury was created with the ligation of left anterior descending coronary artery lasting 30 minutes and postischemic reperfusion persisted 60 minutes also.4 We observed the incidence of reperfusion arrhythmias and the rasing ∑ ST of ECG (lead Ⅱ) after loosening ligature it. Ten rats of each team were collected blood samples from carotid artery, taking serum apart, tested serum for detecting CK ( Creatine kinase ) and LDH ( Lactic dehydrogenase ), and were killed and took the myocardial tissues for detecting the activities of ATPase (adenosine triphosphate enzyme); the other tens of each team were took the myocardial tissues for detecting the level of Ca2+. Two myocardial samples each group at random were took for observing their structures under a electron-microscope.Result1 Compared with group Ⅰ, the incidence of reperfusion arrhythmias, the rasing ∑ST of ECG, the level of serum CK and LDH, the content level of Ca2+ in myocardial tissues of group Ⅱ were much higher(P< 0.01). While the activities of Na+-K+-ATPase and Ca2+-ATPase in myocardial tissues of the same group were much lower (P< 0.01).2 Compared with group Ⅱ, the incidence of reperfusion arrhythmias, the rasing E ST of ECG, the level of serum CK and LDH, the content level of Ca2+ in myocardial tissues of group Ⅱ were much lower(P < 0.05 or 0.01). While the activities of Na+-K+-ATPase and Ca2+-ATPase in myocardial tissues of the same group were much higher (P < 0.01).3 Compared with group Ⅲ, the incidence of reperfusion arrhythmias, the rasing ∑ ST of ECG of group Ⅳ and the content level of Ca2+ weren't significantly different (P > 0.05). The level of serum CK and LDH were much higher (P < 0.05 or 0.01), the activities of Na+-K+-ATPase and Ca2+-ATPase in myocardial tissues were much lower (P< 0.01).4 Compared with group Ⅲ, the incidence of reperfusion arrhythmias, the rasing ∑ST of ECG of group Ⅴ, the level of serum LDH and the content level of Ca2+ weren't significantly different (P > 0.05). The level of serum CK were much higher (P<0.05 or 0.01), the activities of Na+-K+-ATPase and Ca2+-ATPase in myocardial tissues were much lower (P < 0.01).5 Comparing group Ⅳ with group Ⅴ, the incidence of reperfusion arrhythmias, the rasing ∑ST of ECG, the level of serum CK and LDH, the content level of Ca2+, the activities of Na+-K+-ATPase and Ca2+-ATPase in myocardial tissues etc. weren't significantly different (P > 0.05),.6 Oberved through a electron-microscope, the myocardial ultrastructures of rats of group Ⅰ was normal, while those of group Ⅱ were the worst damaged among five groups. The damage of group Ⅲ, Ⅳ and Ⅴ was less than group Ⅱ.ConclusionJinXiangDan possesses protective effects on experimental myocardial ischemia-reperfusion injury in rats, and is superior to DiAoXinXueKang, its effect is directly proportional to dosage within certain range. The results suggested that JinXiangDan can prevent Calcium-overload, protect ultratructure of myocardial cells and diminish cardiac arrhythmia, can also increase the activities of ATPase and... |
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