| ObjectiveThrough observing the effect of Fufangkuqiaomai (FFKQM) decoction on the body weight, kidney weight, index of kidney hypertrophy (kidney weight/ body weight), blood glucose, serum lipid (total cholesterin, triglyceride, high density lipoprotein, low density lipoprotein), kidney function (serum creatinine, blood urea nitrogen), urinary micro-albumin excretion (UAE), expression of platelet-derived growth factor-B (PDGF-B), tumor necrosis factor-α (TNF-α) and accumulation of fibronectin (FN) in kidney cortex on diabetic rats induced by injecting streptozotocin (STZ) into abdomen, to investigate the acting mechanism of FFKQM decoction and provide an experimental basis for clinical medicating and for future developing FFKQM. MethodsThe 61 male Wistar rats were randomly divided into 2 groups: 11 rats were in the normal control group, the rest 50 rats were in another group, which were all induced into diabetes mellitus (DM) by injecting STZ into abdomens once. After 72 hours those rats whose blood glucose was up 16.7mmol/L were into DM group, and were randomly divided into 4 groups. So they were high dose group, low dose group, xiaokewan group, pathology group, and were stomach fed with high dose FFKQM decoction, low dose FFKQM decoction, xiaokewan suspension and normal saline respectively every day. The normal group was stomach fed with normal saline, too. During the treatment, we observed their general condition, such as eating, drinking, excretion and so on. 8 weeks later, we collected their urine. After killed, their blood was collected and tested the blood glucose, TC, TG, LDL, HDL, Scr and BUN. And all rats were tested body weight, kidney weight, UAE, and were observed the expression of PDGF-B, TNF-a and FN in kidney cortex by immunofluorescence technique. All data was analyzed by the soft of SPSS 11.0 for windows. Results1 Body weight There were no significant differences among all groups before treatment, P>0.05. After 8 weeks, the body weight of pathology group kept on decreasing. The body weight of treated groups were higher than that of the pathology group, p<0.01.The effect of the high dose group was better.2 Blood glucose Before treatment there was no distinct differences between therapeutic groups and pathology group, P>0.05. But the level of blood glucose in DM groups were higher than that of the normal group, p<0.01. After treatment the level of blood glucose in therapeutic groups was lower than that in pathology group before treatment, p<0.05 or p<0.01, and the high dose group had better effect on lowing the level of blood glucose.3 Serum lipid (1)Total cholesterin(TC): The level of TC in therapeutic groups was lower than that in pathology group, p<0.01, and the effect of the high dose group wasbest in all treated groups; (2)Triglyceride(TG): The level of TG in all therapeutic groups was distinctly lower than that in pathology group, p<0.01; (3)Low density lipoprotein(LDL): Compared with pathology group, the level of LDL in all therapeutic groups was lower, p<0.01, and the high dose group had the best effect among them; (4)High density lipoprotein(HDL): The level of HDL in all treated group was higher than that in pathology group, p<0.01, and the high dose group had the best effect among them.4 Kidney function(Scr and BUN) There were no significant differences among all groups, P>0.05.5 Kidney weight and index of kidney hypertrophy Compared with normal group, kidney weight and index of kidney hypertrophy in pathology group were higher, p< 0.01; Kidney weight and index of kidney hypertrophy in all therapeutic groups were lower than those in pathology group, p<0.01, and the high dose group had the best effect among them.6 Urine micro-albumin excretion(UAE) Compared with normal group, the level of UAE in pathology group, low dose group and xiaokewan group was significantly higher, p<0.01, and there was no distinct differences between high dose group and normal group; The level of UAE in all therapeutic groups was significantly low... |
PDF Full Text Request