Pharmacodynamics And Mechanism Of Tongmai Particle Treatment Of Puromycin Aminonucleoside Nephropathy Is Discussed

Nephropathy Syndrome(NS) is a worldwide disease with a high incidence anddose harm to human physical and mental health. There are four clinical typesincluding a great deal of albuminuria, low albumen blood diease, high greasediease and apparent dropay. In the aspect of clinical therapy, hormone andimmunosuppressant including cortin, cell toxin, biological preparation are themost of the medicine commonly used in the western medicine to treat NS at theprecent. Due to the biggist toxin and side effect of incretion andimmunosuppressantrelatively looger using drug, and quite quabtitative suffers arenot sensitivity to the therapy. So exploring new approachs of treatment has manyimportant clinical values. Chinese traditional medicine has orginal departments inthe treatment of NS. At the present inside and outside of the country it had beenget the hightly attention. Empoldering and investigating new types of Chinesetraditional medicine to treat NS has become the hotapot in our research. Tongmai Granule (TM), is used as an effective Chinese traditional medicineclinically in the hospital belong to the Guangzhou University of Chinese traditionalmedicine. In order to provide the experimental basis for the clinical using, we havestudied the suppressive effect of TM on the model induced by puromycin aminonucleoside(PAN). In the part of paper, we expatiated on the knowledge of the name, pathogeny,pathogenesis, treatment, prescription of NS in the literatures of ancient Chinesetraditional medicine, and correlative knowledge of modern medicine. Then we carried out the following experiments: 1. Establishing PAN-treatedmodel. 2. The preventive effects of Tong-mai Granule on the model induced byPAN were evaluated. Sixty rats were divided into six groups. They are normalcontrol group,PAN-model group,Tong-mai Granule group of high, middle, lowdosage, ACE group, Urine samples were collected from the rats in metabolic cageson days 5, 10, 15, 20, 25 and 30 after the injection of PAN. All rats were sacrificed31 days after the injection of PAN for blood biochemistry, RNA preparation, lightmicroscopy and electrical microscopy of the kidneys. 3 , We assayed theexperission of Bone morphogenetic protein(BMPs) and intracellular protein-Smadin the kidney. Our study indicates that we make the PAN-model successfully.The results ofthirty day's observation showed that Tong-mai Granule could decrease urinaryprotein excretion, regulate abnormal blood fat metabolism, save renal function andimprove pathological changes in PAN model rats, inhibit of expression of BMPRIIand Smad1mRNA in the kidneys.