| Kawasaki disease (KD)as known as mucocutaneous lymphnode syndrome (MCLS)is an acute febrile systemic vasculitis primarily affecting illness hat occurs in young children. The clinical features of the acute phase of the symptoms are fever, rash, changes of skin and mocosa and nonsuppurative cervical lymphadenopathy and so on. The danger of potential is severe stenosis or complete coronary artery occlusion for coronary arteries. The main cause of death is myocardial infarction. The disease incidence gradually increased from 1970 to the late 1990s. Now, It becomes one of the most common diseases of acquired heart diseases in young children.Despite intensive research, its etiology and pathgenesy remain unknown. With the rapid development of immunology, a great breakout research has made in. The superantign hypothesis has revealed the secret that many scholars have not searchedsingle pathogen from patients with Kawasaki disease to make use of serology and advanced cultivate skill. Immune functional disorder plays a very important role in Kawasaki disease. There is unusual activation in immune system of Kawasaki disease that distinguish with other diseases.T lymphocyte act as very important role in immuological regulation, its main functions include activation, multiplication, clonal expansion, cell differentiation, apoptosis and immunological memory. T lymphocyte activation is an important ingredient in immunoregulation. Conflicting data have been reportded regarding T cell activation in peripheral blood during acute KD. Recently, Exterior and interior scholars have paid close attention to CD69, CD25 and HLA-DR as marks of T lymphocyte activation. However, whether or not peripheral blood T cells are activated in patients with acute KD remains uncertain. To the best of our knowledge, we are the first to analyse CD69 expression in peripheral blood T cell in KD patients.This content is to study the changes of CD69, CD25 and HLA-DR expression in peripheral blood T cell in Kawasaki disease. In this study, we detected CD69, CD25 and HLA-DR expression in peripheral blood T cell by flow cytomery. It can show the respondent intentsity and to detect multparameter on the level of cells and subsets. It is worthwhile to understand the T cell activation in peripheral blood T cells in patients with acute KD. The changes of peripheral blood T cells activation have an very important significance to interpret the pathgenesy, the process of disease and immunology treatment.[materials and Methods]1. Cases and groupsKawasaki disease group: the patients who met the diagnostic criteria for KD comprised sixteen boys and fifteen girls (4~60 months of age; mean, 26.23±17.79 months). All received intravenous gammaglobulin (i.v.GG), lg/kg/day, for 2 days and oral aspirin (30~50mg/kg/d). The onset of illness was defined as the day on which fever appeared. Blood samples were obtained prior to treatment.Healthy control: we tested samples from 17 healthy controls consisting of nine boys and eight girls (3~84 months of age; mean, 24.76±18.17 months)in parallel with the samples from the patients.Upper respiratory infection (URI)group: we tested samples from 8 URI patients whom blood CRP were normal, consisting of four boys and four girls (12~34 months of age; mean, 19. 25 ±7. 80 months)in parallel with the samples from thepatients.KD self-control: this study included fifth boys and three girls (21~42months; mean, 29.63±6.80 months).2. Measurement of CD69, CD25 and HLA-DRWe detected CD69, CD25 and HLA-DR expression in peripheral blood T cell by flow cytomery. Whole blood (30ul)was stained with fluorescein isothiocyanate (FITC)- conjugated anti-CD3 monoclonal antibodies (mo Ab) (Becton-Dickinson, Mountain View, CA, USA). Erythrocytes were incubatd with 1 ml of lysing solution(Becton- Dickinson)for 20 min. After washing with phosphate-bufferde saline containing 0.5% bovine serum albumin and 0.1% NaN3 (washing buffer)leucocytes were suspended in FACS Permeabilizing Solution (Becton-Dickinson)for 10 min. The cells were stained with phycoerythrin (PE)-conj... |
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