Paul Lung Dingchuantang On The Role Of Chronic Obstructive Pulmonary Disease Airway Remodeling In Rats Intervention

Background: In recent years, the morbidity and disability rate of chronic obstructive pulmonary disease (COPD) are rising. In terms of the prediction of WHO, COPD will be the 5th costly disease in 2020. COPD is a disease state characterized by airflow limitation that is not fully reversible, usually progressive, and associated with an abnormal inflammatory response of the lungs caused by harmful particles and gases. The stimulations of chronic inflammation and incompletely repair lead to the abnormality of the structure and function of the airway, including the proliferation and hypertrophy of smooth muscle cells, the multiplication of fibroblast, the deposit of collagen and amino-polysaccharide, the proliferation and hypertrophy of goblet cells and mucous glands whose secretion rise, and so on. All of these lead to the remodeling of airway, at the sametime, the inflammation and remodeling of airway will quicken the process of irreversible airway obstruction. It is sure that the pulmonary vessels take part in the process of remodeling . The active fraction of pulmonary vessel such as growth factor, ET-1, may impact the mechanism of COPD in molecule level, the effects of growth factor in the deposition of extracellular matrix and the multiplication of smooth muscle cells is more appealing. Recently, The scholars abroad start to research the effects of vascular endothelial growth factor(VEGF) in the remodeling of airway and vessels. On the other hand, at present, there is lack of effective and safe medicine for stationary phase. It is necessary to do some advanced research to make sure whether Bao Fei Ding Chuan Tang for supplementing qi, promoting blood circulation, clearing lung heat and resolving phlegm is effective to adjust the expression of VEGF in the tissues of lung and bronchia and intervenee the remodeling of the airway. At first, we should built an ideal animal model. At present, the method of building the rat model of COPD, which is similar with human and characterized by remodeling of airway is exposure to cigarette smoking and LPS dropped in airways. Objective: To observe the effects of Bao Fei Ding Chuan Tang on the expression ofVEGF and remodeling of airway structure in COPD model of rats, and further study the mechanism of famous doctors' experience formula, try to develop a new medicine to prevent COPD.Methods: 1. 40 male healthy SD rates (160±20g) were randomly divided into a normal control group, a model group, a prevention group and a treatment group( with Bao Fei Ding Chuan Tang). 2.The rats of model group, prevention group and treatment group had been exposed in cigarette smoking for 10weeks, every rat was dropped LPS200u g into airway on the first day and the 28th day . 3.The rats of the prevention group were fed with 9.3g /kg of the Chinese medicine from the 7th day before molding , the rats of treatment group were fed with 9.3g /kg of medicine from 29th day after molding. 4.To fix the right lower lobe of lung by 10% formalin, then do HE straining, Masson straining and immunohistochemistry straining. To measure the length basement membrane ,the total area and diameter of airway wall, the average number and area of alveoli, the area of collagen in airway wall and area of VEGF expression in the bronchiole and pulmonary artery. 5.Extracted the total RNA from right middle lobe of lung and to detect the expression of VEGF mRNA With RT-PCR. 6.To analyze data with SPSS 11.5.Results: 1. Compared with normal control group, the rats of the model group and treatment group grew up slowly. Bao Fei Ding Chuan Tang could prevent the rats suffering from COPD from loss of bodyweight. 2. with light microscope , in model group, bronchiolar cilia adhesived and prostrated, some epithelium cells shedding and damaged, goblet cell hyperplasiaed, lymphocytes and macrophages infiltrated in the submucous membrane and muscle. Bronchiole dilated, duct wall thickened, the intervals between lung cells and broke alveolar dilated. The infiltration of lymphocytes reduced significantly in the prevention group and treatment group.