Inbred Department Of Rat Osteosarcoma Fischer344 Transplantation Model In The Establishment And Caffeine On The Synergies Of The Fischer 344 Rat Osteosarcoma Chemotherapy

Transplanted model of osteosarcoma originated form inbred strain Fischer 344/N rat is a easily got model with excellent success rate. And it has normal immune system, that it can duplicated the in vivo immune circumstance of real tumor. Methods 24 male Fischer 344 rats, weighted about 200g (199.36 ± 7. 27g), were equally divided into 3 groups randomly. 0.5ml S-SLM cell, in the concetration of 3.0x 106, was injected into the subcutaneus of rats in group A. 0.5ml S-SLM cell, in the concetration of 3.0x106, was injected into the peritoneum. And 0.5cmx0.5cmx0.5cm size tumor tissue, got from the peritoneual injected tumor, was transplanted into subcutaneus of group C. Measure the dimension of the subcutaneus tumor every 7 days, evaluated the tumor weight, and record the survive days, then kill all rats in 10 weeks, check the lung metastasis in global andmicroscope. Resu I ts 2 rats in group A were found tumor grow in subcutaneus(25%,2/8), metastasis rate was 0. All 8 rats in group B were found ascite forming in 15-20 days after injection of S-SLM, tumor grew in the peritoneum, but no lung metastasis was found. And tumor grew well after transplanted to subcutaneus in all 8 rats in group C, and lung metastasis were discovered in 7 rats, the metastasis rate was 87.5%(7/8). Conclusion The way of transplant the peritoneum S-SLM cell injected-tumor to the subcutaneus of Fischer 344 rats is a successful method to creat a easily got, economical animal model of osteosarcoma, with high success rate.