| Objective: To investigate the inhibition effect of DADS on SW480 cell line in vitro and its related mechanism.Methods: The growth inhibition of SW480 cells was measured by growth curve analysis ,vitality detection and MTT assay. Cell morphology was observed by optics microscope and electron microscope. Phase distribution of cell cycle was analyzed by flow cytometry (FCM). Immunocytochemical staining and morphometric quantitative analysis detected the expression of PCNA, p53, p21WAF1, Bcl-2 and Bax. Results: MTT assay showed that DADS from 30 to 70 μg/ml significantly inhibited SW480 cells and exhibited a dose-dependent modal. After exposure to DADS, cell viability of SW480 decreased from controls 97.37% to experimental 80.83% (P<0.05), average doubling time retarded from 34.50 hours in normal cultured SW480 cells to DADS experimented SW480 cells 94.74 hours (P<0.05). As exposed to optics microscope and electron microscope, SW480 cells took on malignance declining as cellular heteromorphism diminished, nucleocytoplasmic proportion was reliable to reasonableness, cellular apparatus were abundant in plasm ,nuclear and partial cell organs manifest retrograde alters and partial cells manifest apoptosis morphologic alters;All these showed above suggested that SW480 cells malignancy and proliferation capacity is declined DADS. Flow cytometry analysis revealed that the cell content of G1-phase declined whereas G2-phase increased after exposure to DADS , which indicated that colon cancer cells arrested in G2-phase (P<0.05) ;Hhyodiplod peak is higher,which means cells apoptosis induced by DADS. Immunocytochemical stain and morphometricquantitative analysis indicated that expression of p53, PCNA, Bcl-2 reduced andthat expression of p21 WAF1 and Bax enhanced (PO.05) .Contusion1. To SW480 cell line, DADS has significance growth inhibition and this effect is a dose-dependent and time-dependent model.2. DADS induce SW480 cell block in G2/M and apoptosis.3. The growth inhibition and the cell blocked in G2/M of DADS in human colon cancer SW480 cell line probably relate to downregulated expressions of p53, PCNA, Bcl-2 and upregulated expression of p21WAF1 and Bax.Objective: To investigate the inhibition proliferation and apoptosis effect of DADS against SW480 cells in vivo and its underlying mechanism. Methods: A total of 20 nude mice were randomly divided into 4 groups: control, therapy, prevention and treatment group in vitro. Treatment group was inoculated with SW480 cells treated 48 hours by DADS. Control, prevention and therapy groups were inoculated with SW480 cells. Prevention and therapy group were administrated with DADS through abdominal cavity respectively from the second day and after the tumor formed. Control group was injected with NS after the tumor formed. Transplant tumor's weight and volume were observed; morphologic change was analyzed by optical microscope and electron microscope. Phase distribution of cell cycle was analyzed by flow cytometry (FCM). Immunocytochemical staining and morphometric quantitative analysis detected the expression of PCNA, p53, p21WAF1, Bcl-2 and Bax.Results: SW480 cell xenograft tumor model was successfully established. The rate of tumor formation of treatment, prevention group were about 40% , 60%, respectively, whereas the control group was 100%,which demonstrated that the SW480 cell oncogenicity was decreased sharply by DADS, and DADS prevented human colon cancer occurrence in vivo. The volume and weight of transplant tumor of therapy group were significantly decreased than that of control group (P<0.05), which showed DADS markedly inhibited the growth of transplant tumor. The antineoplastic rate of therapy group, prevention group and treatment group in vitro was 62.86%, 80.94%, 87.64%, respectively. Cellular atypia and nuclear-cytoplasmic ratio were reduced, cytoplasmic cell organs were abundant, nuclear and partial cell organs manifested retrograde alters and partial cells... |
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