| This research's order is to isolate lactic acid bacteria that can produce bactericin and lactic acid during fermention from the vagina of 60 healthy women. The 19 strains of bacilliform bacteria were identified by biochemical tests, part of their 16S ribosomal DNA were amplified, sequenced, and phylogenetically placed. The results showed that the lactic acid bacteria were belongs to those special homotype fermention types of Lactobacillus.9 strains belong to Lactobacillus gasseri, one does Lactobacillus.salivarius and the other 9strains do Lactobacillus.crispatus. They could produce lactic acid , bactericin ,and H2O2 except 3 of them,remaining the acid circumstances and inhibit the growing of the other pathogentic bacteria, such as Staphylococcus aureus, Escherichia.coli etc. They could not inhibit the spherical bacteria that are isolated from the same circumstance and themselves except one strain 46-2. So these Lactobacillus strains that are isolated from the bodies have several effective actions to keep the vaginal healthy balance. We should use these Lactobacillus strains to make thep robiotics, according to the humanity current of the vaginal health protection. |