Xuanfei Dingchuantang Inhibitory Effect Of Rat Airway Remodeling And Inflammatory Mediators Of Asthma

ObjectXuanfeidingchuan Decoction is the effective prescription which is basing of many years of clinical practice of advisor which was summing up experience from the treatment of asthma. It is the adding-deducting prescription of Xiao Qing Long Decoction and was expounded in the "Treatise on Cold-Induced Diseases" and "Synopsis of the Golden Chamber". The Xiaoqinglong decoction apply for the asthma of exogenous cold and internal retention of morbid fluid, the group follow the principles for the treatment of asthma remedy of ventilating lung to dissipat morbid fluid and relieving cough to relieve asthma ang it is the best prescription for asthma. This experiment aimed at exploring depressant effect to airway Remodeling and inflamed medium of asthma rat. It open up a new avenue of preventing and curing asthma for classical prescription, and for the development of antasthmatic drug Xuanfeidingchuan Decoction as antasthmatic drug provide objective basis. at the same time in the theory of Chinese medicine therapeutical principle,and modern pharmacological research in asthma have a practical significance.MethodsThrough literature studying status quo of Xiao Qing Long Decoction treating asthma, and on this basis, through preliminary experiments revealed depressant effect to airway Remodeling and inflamed medium of asthma rat of Xuanfeidingchuan Decoction, the specific methods as follows:1, The research of Xuanfeidingchuan Decoction effect the lung bronchus and morph of rat. The experiment use optical microscopes to observe asthma rat lungs organizations morphology changes, and analysis through the using of the medical system, measuring bronchial fundament circuit (Pbm), the total vessel wall area (WAt), the inner wall area(WAi) and smooth muscle area (WAm), and standardize the measurements with Pbm and through WAt/Pbm, WAi/ Pbm, WAm/ Pbm to express thickness that each of the corresponding wall, and Observer airway Remodeling of lungs organizations of asthma rat.2,The effect of Xuanfeidingchuan Decoction on the inflamed cell expression levels of BALF and blood of asthma rat.3,The effect of Xuanfeidingchuan Decoction on IgE expression levels of blood of asthma rat by using the method of radiation immunization.4,The effect of Xuanfeidingchuan Decoction on Eotaxin expression levels of BALF and lungs organizations of asthma rat by using the method of ELISA and immuohistochemistry.5,The effect of Xuanfeidingchuan Decoction on ICAM-1 expression levels of BALF and lungs organizations of asthma rat by using the method of ELISA and immuohistochemistry.Result1, Discovering that Xuanfeidingchuan Decoction groups can relieve inflamed cells sinfiltrating and the thickening of bronchus smooth muscles obviously compared with patho-model group through through optical mirror observation of asthma rats...