Learning And Memory Dysfunction In The Rat Brain Of Apoe Ginkgo Biloba Extract On Diabetes, Gfap Expression

Objective: In the present study with streptozotocin-induced diabetes in Wistar rats as objects, we have investigated the protective effects of EGb on brain in behavior profiles and molecular biology aspects.Methods: The diabetic rats model were produced by injection 55mg/Kg streptozotocin, we observed the behavior profiles by open field test, and the learning and memory potential by Morris water maze , RT-PCR was used to detect mRNA expressions of ApoE,GFAP in hippocampus and cerebral cortex of diabetic rats with learning and memory functions deficit.Results:①The result of open field test showed that the center detention time was prolonged ,accompanied by decreased numbers of hollow-probing in diabetic rats .②The result of Morris water maze showed that the escaping latency was prolonged, and score of platform-searching was decreased on d5 and d8. EGb administration (100, 50 mg/kg ) could shorten center detention time, increase numbers of hollow-probing, improve deficient escaping latency and score of platform-searching in diabetic rats.③The result of RT-PCR showed that the mRNA expression of ApoE in hippocampus of diabetic group was higher than that of control group, but not evidently changed in cerebral cortex(P> 0.05). The mRNA expression of ApoE in hippocampus of EGb (100,50mg/kg) administration were lower than that of diabetic group,but not evidently changed in cerebral cortex(P> 0.05). EGb could inhibit significantly the mRNA expression of ApoE in hippocampus of diabetic rats with learning and memory functions deficit.④The result of RT-PCR showed that the mRNA expression of GFAP in hippocampus of diabetic group was increased significantly, compared to the control group, but not evidently changed in cerebral cortex(P> 0.05). The mRNA expression of GFAP in hippocampus of EGb (100,50mg/kg) administration were decreased significantly, compared to...