Allergic Rhinitis Mouse Sinus Mucosal Bacterial Adhesion And Nf-¦Êb P65 And Glucocorticoid Receptor Expression

Objective To develop a mouse model of bacterial rhinosinusitis superposed on allergic rhinitis (AR), and explore whether ongoing allergic rhinitis enhances the acute sinus infection and inflammation associated with Streptococcus pneumoniae. Methods 40 mice of C57BL6/J were randomly divided on average into 4 groups: A (OVA+Sp), B(OVA+NS), C(PBS+ Sp) and D(PBS+NS) .①Group A and B were sensitized by intraperitoneal injection 200μl(10%)OVA on days 0 through 8, and exposed to OVA(6%) intranasally on days 9 through 16, as to induce allergic inflammation. PBS was used in group C and D in the same way.②Subsequently, group A and C were inoculated Sp(1.2×109CFU/ml) intranasally on day 12, and NS was used in group B and D. On the 6th day after inoculation, mice were killed. Blood was gathered from the orbital venous sinus after anesthesia. The heads were embedded with paraffin and serial sections were made and stained with hematoxlin-eosin and toluidine blue for histological analysis,inflammation cells count. The number of polymorphounclear neutrophils and eosinophils per square millimeter of sinus mucosa (PMN/mm2 and Eos/ mm2) were calculated by using a computer-aided special software under microscope. Results AR models were successfully established in 9 animals from group A and 8 from group B. Histology examination of the sinus from group A and B revealed significant mucosal edema and dilated venules. The symptoms were mild in group C, and no symptom was observed in group D. PMN/mm2 in group A(139.29±26.51)mm2, was significantly higher than that in group B(70.67±16.74)mm2, C(63.04±14.73)mm2 and D(40.24±14.05)mm2 respectively(P<0.01); Eos/ mm2 and serous IL-5 level in group A(134.63±25.49)mm2(,48.21±13.94)pg/ml and B(116.21±25.17)mm2,(40.83±7.78)pg/ml, were higher than that in group C(16.71±2.68)mm2,(23.89±8.65)pg/ml(P<0.05)and D(13.39±4.95)mm2,(24.58±6.50)pg/ml(P<0.05). Conclusion Our data demonstrate that an ongoing local allergic response augments bacterial infection in mice, and allergic sensitization alone without Streptococcus pneumoniae does not induce the sinus infection.