Ea Du Channel Points Of Focal Cerebral Ischemia And Reperfusion In Rat Brain Gene Expression Profiles

OBJECTIVE: The aims of this study were to investigate the changes of the cerebral gene expression in ischemia and electroacupuncture(EA)-treated ischemia. METHODS: A focal cerebral ischemia/reperfusion rat model was performed with a modified middle cerebral artery occlusion method. Total RNA was extracted from the injured hemisphere at 24 hr after 2-hr transient middle cerebral artery occlusion (MCAo) and gene expression was analyzed by GeneChip. RESULTS: Among the nearly 5,700 genes,175 genes were affected after ischemia/reperfusion injury, in which 115 genes were upregulated, and 60 genes were downregulated. In EA-treated rats, 74 genes were affected, in which 26 genes were upregulated , and 48 genes were downregulated. CONCLUSION: These results suggest that some genes may be involved in the pathologic mechanisms of cerebral ischemia/reperfusion and the therapy mechanisms of acupuncture anti-ischemic/reperfusion injury. Microarray analysis may provide a framework for understanding these complicated mechanisms.