Xie Xin Tang Anti-toxin Effective Components And Absorption Kinetics,

Decoction for purging heart-fire, whose Chinese name is Xiexin Decoction, is a famous prescription mentioned in Jinkui Yaolue (Medical Treasures of the Golden Chamber). The decoction is made up of Radix et Rhizoma Rhei, Rhizoma Coptidis, and Radix Scutellariae, with function of purging fire and detoxifying, heat-clearing and damp-drying. Now, it is employed to treat infectious diseases in clinic.The modern medicine has explained the relations between the infectious diseases' causes, processes, and consequences, and the inflammation induced by endotoxin (ET). According to theories of Traditional Chinese Medicine (TCM), ET is the representative of heat-toxin and pestilential poison, so the followed principles of treatment should be consistent with these theories, which are clearing and descending heat-toxin, supporting healthy energy to eliminate evils. But, the existing researches on Xiexin Decoction seldom follow these theories well, nor explore its fundamental substance or functional mechanism of clearing away heat-toxin and toxic material from a point of view of effective fraction. This article has carried out an integrative research based on theories of TCM, which focused on the effective fraction with anti-endotoxin activity of Xiexin decoction (EFX). The contents are as follows:1. The acknowledgement of EFXDismemberment study had been carried out including measuring and comparing some pharmacological indexes of anti-endotoxin activities. As a result, both whole ingredients and different ingredient groups had certain effects of anti-endotoxin at the different degree, and the effect of the ingredient group composed of Radix et Rhizoma Rhei and Radix Scutellariae exhibited better than the whole ingredients. Further researches on activities of anti-endotoxin in its different solvent extracting fraction showed that n-BuOH fraction had obvious antagonistic effect. Pre-experiment showed that fraction contained anthraquinones in Radix et Rhizoma Rhei and flavonoids in Radix Scutellariae. So it could be confirmed that these are main effective fractions in Xiexin Decoction.Then, the extractions with anti-endotoxin activity were extracted, isolated, and purified, and the proportion of them had been screening by pharmacological experiments. The result showed that compatibility of anthraquinones in Radix et Rhizoma Rhei and flavonoids in Radix Scutellariae exhibited the strongest activity...