Blood Rehabilitation Expression Of Bcr / Abl And Jwa In K562 Cells

Chronic myeloid leukemia (CML) is a hematopoietic disorder characterized by the malignant expansion of bone marrow stem cells. Its cytogenetic hallmark is a reciprocal t(9;22)(q34;q11) chromosomal translocation that creates a derivative 9q~+ and a small 22q~-, known as the Philadelphia (Ph) chromosome~1. The latter harbors the bcr-abl fusion gene encoding a chimeric bcr-abl protein with a deregulated tyrosine kinase activity, the expression of which has been shown to be necessary and sufficient for the transformed phenotype of CML cells.Xue Fu Kang is a new Chinese medicine, which has been used to treat CML for more than a decade in Jiang Su province hospital of TCM. It is effective and safety, and also well-tolerated by most patients. Previous study of our group showed that on a cellular basis, Xue Fu Kang inhibited cell proliferation and induced apoptosis. This paper would further investigate molecular mechanisms of this medicine.Objective: To study the effects of Xue Fu Kang on proliferation and apoptosis of K562 cells, as well as on expression of bcr/abl and JWA mRNA.Methods: K562 cells were handled with different concentrations ofXue Fu Kang(0mg/ml, 2.5mg/ml, 5mg/ml, 7.5mg/ml, 10mg/ml, 20mg/ml) and were harvested in 24 hours. Then morphological changes...