Blood, Raman Spectroscopy And Fluorescence Spectra Of Several New Phenomena

The fluorescence spectra of diluted mouse blood, mouse red cells suspending liquid, red cell hemolysates at different concentration excited by incoherent monochromatic light of different wavelength are investigated experimentally and theoretically in this paper.The fluorescence spectra of mouse blood are investigated by multichannel Fluorolog3-P spectrofluorometor. A remarkable relationship existed between intensity of spectra excited at 407 run and the mouse blood solution at different concentration. Especially at the range from 430 nm to 540 run, a strong and serious variation fluorescence spectra can been observed. It is considered that the relationship is resulted from concentration quenching and the absorption of the blood cells in theoretical analysis. The fluorescence spectra of diluted blood, mouse red cells suspending liquid, red cell hemolysates of blood sample with variation of concentration excitated by monochromatic source of different wavelength. The structure of fluorescence spectra in this experiment have been analyzed theoretically. The spectrum observed at 473 nm is a Raman spectrum, which Raman configuration is more prominence when the diluted blood concentration is less than 1%. Fluorescence emission of characteristic peaks of free protoporphyrin, zinc protoporphyrin, fluorescent heme degradation product and erythrocyte membrane are identified and simultaneously detected in mouse red cell hemolysates, when excitation light is 488 nm. It has been observed that there exist red-shifted in zinc protoporphyrin characteristic spectra of diluted blood and red blood hemolysates with the concentration increase from 1% to 10%. The phenomenon has been analyzed and explained later.In this paper fluorescence spectrum of blood sample excitated at 457 nm Xenon light have been compared with other sources such as Ar~+ laser (λ=457.9 nm) and LED light (1=457 nm), there are difference among the spectra structures. The explanation has been give elementarily.The research in this paper can offer reference to mechanism of interaction between light and blood, the optical biological effect and clinic diagnose technology in virtue of spectroscopy.