Growth Of Human Hepatocellular Carcinoma In Situ Transplantation Resistance Model

Objective: To establish a multidrug resistance (MDR) model of orthotopic transplantation of human hepatocellular carcinoma (HCC) in nude mice by intermittent abdominal chemotherapy.Methods: The hepatocellular carcinoma cell line HepG2 was first cultured in vitro, then subcutaneous carcinoma was produced to form the tumor donor mice. An orthotopic mdrl hepatoma was produced by implanting the tumor fragment under liver capsule and adriamycin (ADM 1. 5 mg/kg, once a week for 8 weeks) was treated by ip to abdominal cavity of nude mice. The cytotoxicity to cancer cells was determined by MTT assay. RT-PCR and FCM were performed to detect the expression of mdrl-mRNA and P-gp protein.Results: The typical behaviors of HCC were observed in the model. The expressions of mdrl-mRNA and P-gp in the ADM-treated group were higher than that of the control group. The IC50 of ADM-treated group was 15.6 times higher than that of non ADM-treated group. The drug-pump-efflux function of P-gp on the cytomembrane of hepatocellular carcinoma cells with positive and mdrl gene expression was enhanced significantly.Conclusion: The multidrug resistance model of orthotopic transplantation of human HCC was established successfully. It could provide a reliable model for exploring the mechanism of multidrug resistance and reversing multidrug resistance in HCC.