| Varicella-pox- zoster virus (VZV), belong to the a—herpes virus subfamily , be person herpes virus 3 types. That hair infection activates whose plain once again for Varicella-pox, lurks is to arouse a zoster. Great majority are all self's life immune , also having a handful of people will be after VZV infects the human body at ease, be affected by once again. Individual have suffered from Varicella-pox previously, think that accepting the immune restraint queen is able to show the representative zoster. In recent years, zoster morbidity has neuralgia to increase the high trend obviously, and the queen who arouses loses to use antiviral medication to there be no the obvious curative effect. Already gradually, this two kinds diseases and their relevance sequela becomes the important public health problem but is regarded highly gradually. In the field of the vaccine, the lively vaccine of Varicella-pox attenuation is unique obtain permission to be used to take precautions against the vaccine from the disease that human being herpes virus arouses. Although, the vaccine immunity effect has got a dead cert, there exists some are be worth studying further and perfect problem in the vaccine in Varicella-pox in inoculating with a job. The problem the Varicella-pox herpes virus is carried out parting for and cultivating and carries out an appraisal mainly by the cell cultivation method, the virus sample book can be used for Varicella-pox- zoster vaccine research , also, can come on for Northwest Territories the days to come Varicella-pox- zoster and prevent from establish the certain basis.Varicella-pox- zoster virus separates success as far back as 1954 years , that whose natural infection happens only makes the person study slowly in person and the gorilla since but whose altitude species belongs to peculiarity. Use the method coming to separate a virus extracorporeal culture to be relatively mature at present, the liquid collecting the inner to fresh herpes of patient inoculate with carrying out passage among living cell, expand the virus being adapted to cell cultivation increase a virus , final unceasingly. The cell cultivation studying serum, uses Vero from the clinical have collected herpes liquid and impatient scheduled time of patient, convalecent stage originally arrives at 2 dwarf viruses from parting for from 9 in example Varicella-pox and zoster patient Varicella-pox liquid. 2 virus has representative VZV bureau cooking stove sex cell lesion characteristics such as making the infection cell garden shrink , fusing , coming off equally , CPE increases by but is accelerated with passage number of times. Use peculiarity to resist the VZV antibody has made the indirect immune fluorescence admeasurement toseparation, visible representative infection cell nucleus inner fluorescence piece. Ag movie that 2 virus infection cells are made into, check 4 impatient scheduled time of example zoster patient and convalecent stages serum, the fluorescence antibody has equally increasing height above 4 times. The monthly poison preserving one affects the Vero cell in -20℃, 3 time of passage has not seen CPE appears on cell in a row. Certain probability separates out the Varicella-pox- zoster virus in test result being indicated , being using extracorporeal culture method to be able to from clinical patient bleb liquid, this virus is comparatively sensitive to Vero cell. The experiment separates VZV to 2 determining the pathogene being caused disease by a patient indeed after peculiarity serum antibody. Separate VZV not stabilizing in extracorporeal Biology character, the unable long range preserves in-20℃. That 7 example specimens have not parted for to main cause of virus has been that what be taken blister liquid amounts is very few , the patient had used and antiviral medicine such as interferon , Virazole , graceful past Luowei. |
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