Camellia Shell Active Substance Extraction And Antioxidant Mechanism Of Inhibition Of Fatty Acid Synthase

China is the largest camellia oil production country. Camellia oleifera abel is a special oilspecie which has a history of 2300 years in our country. It is estimated that there are 366,700 hm²Camellia oleifera abel which produce 1,500,000,000kg camellia oil every year but also leave us10,000,000,000kg camellia shell at the same time. So if we can make good use of it we may getmuch benefit and creat more economic profits. But in a long time little effort is devoted toidentify and isolate the minor potential chemopreventive agents present in the shell of camelliaoleifera abel and assess their properties, due to its trace amount and complexity. The objective ofthis study was to isolate and identify the bioactive compounds of the extract of camellia shell andto characterize the antioxidant properties and as a FAS inhibitor of these compounds.The optimum extracting conditions of total flavones from the shell of camellia oleifera abewere studied. The best conditions were chosen through orthographic experiments. The resultsshowed that 30:1 of 60% ethanol was used to douse the dried the shell of camellia oleifera abewith ultrasonic treatment last for 45min twice. The average extraction rate was as high as 97.34%.The content of flavones in the extraction liquid was as high as 1.71%.To evaluate their antioxidant activities, we uses three systems like dpph, frap and·OHscavenging capability. Results shows that in DPPH system the 40% ethanol extract have a goodantioxidant activity, its IC₅₀ is 781μg/mL, lower than bamboo extract which IC₅₀ is 859μg/mL andobviously lower than the other extract; In FRAP system, the FRAP value of water extract is 1.79mmol/L which is very close to bamboo extract but lower than Vc; In·OH scavenging system,when all the extract under the 500μg/mL, scavenging rate for 40% ethanol extract, ethanol extractand bamboo extract is 32.8%, 38.1%, 40.9% respectively, but lower than Vc.HPLC analysis show that camellia shell may include Quercetin and Rutin, which has acontent of 42.5mg/kg and 1478.5mg/kg respectively. Experiments also show that the extract ofcamellia seed shell (CSS) inhibited FAS activity with strong reversible fast-binding inhibition.40% ethanol was the optimal solvent for extraction, half inhibition concentration (IC₅₀) of CSS toFAS overall reaction was 2.02μg/mL. We also found the extract of camellia seed residue (CSR)inhibited FAS activity with irreversible slow-binding inhibition. In the presence of 0.31 mg/mL ofSCR the inhibition led to over 85% activity loss. These results showed that FAS inhibitor in camellia seed had a stronger inhibition ability than the known inhibitors. It is expected to be anew effective FAS inhibitor, and may be of great value for the study of FAS methanism and thecontrol of obesity.