Cistanche On Pig In Vitro Culture And Its Mechanisms Preliminary Study Of The Effect Of Early Embryonic Development

The investigation studied the effect of Cistache deserticola Y.C,Ma using the embryos of pig as explant.Three experiments were set: the effect of Cistache deserticola Y.C,Ma of different concentration on embryos of pig development in vitro ; the effect of Cistache deserticola Y.C,Ma on the embryos early development of pig in different phases;the effect of Cistache deserticola Y.C about NO concentration on embryos early development of pig in vitro .It expect of deploitation a new approach on improve ratio of embryos development of pig in vitro and extend the application range of Chinese traditional medicine.Experiment 1 used the NCSU-23+0.4%BSA as control,supplemented with Cistache deserticola Y.C,Ma of different concentration as tests.After several replicated,4 concentrations were selected to further selection. The result showed that the best concentration of Cistache deserticola Y.C,Ma is 50μg/ml which cleavaged embryos and blastocyst were 85.12±2.9%,31±3.0% was much higher than the control(76.73±3.2%,24.89±4.3%)(P<0.05),there were no big difference about test groups(P>0.05). The NCSU-23+0.4%BSA supplemented with Cistache deserticola Y.C,Ma significant improved the development of embryos in vitro.Experiment 2 The embryos needed different nutritious substance in different phases .In order to definitude which phase was better than other phases when it access Cistache deserticola Y.C,Ma,the experiment used the NCSU-23+0.4%BSA as control,supplemented with Cistache deserticola Y.C,Ma of three phases of embryos development in vitro.The result showed that when supplemented with Cistache deserticola Y.C,Ma of two_four cell phase was best.it's blastocyst(48.72±6.6%)was much Higher than the control(25.08±5.4%)(P<0.01),and it better than IVM and IVC(P<0.05).It is concluded that the NCSU-23+0.4%BSA supplemented with Cistache deserticola Y.C,Ma on two_four cell stage can effective promote the development of embryos in vitro.Experiment 3 The experiment was mensurated NO concentration of embryos until they were developmented at blastocyst stage. Observe the effect of Cistache deserticola Y.C.Ma on embryos of pig about NO concentration.The result showed that the embryos had much NO when they were cultured at blastocyst stage,the control and test were 26.33±1.90 and 20.30±0.95 respectively,but the supplemented with Cistache deserticola Y.C,Ma was lower than control(P<0.05).This indicate that basal culture medium supplemented with Cistache deserticola Y.C.Ma can eliminate free oxygen radical(FOR) which were produced by the embryos in vitro culture and it also can optimize bidirectional adjust effect of NO in cellular signal acess that enhance the ratio of development in vitro.