Silkworm Chrysalis Oil Lipid Levels Of Hyperlipidemic Rats And Non-alcoholic Fatty Liver Formation

Objective: To study the effects of silkworm pupa oil on the serum lipids level and the formation of nonalcoholic fatty liver. Methods: 100 SD rats were randomly divided into 9 groups according to the serum lipids level: normal control group, high-fat control group, positive control group, low, medium and high dose preventive groups, low, medium and high dose therapeutic groups. The relevant indexes about serum lipids level were tested respectively in the first, the sixth and the eleventh week. And the liver index and hepatic TG, TC and MDA level were obtained when the rats were killed in the eleventh week. Moreover, the hepatic pathological sections were made to observe the pathological change of the liver. Results: (1) In the 6^th week, serum TC, TG and LDL-C level of 3 preventive groups were significantly lower than in high-fat control group (p<0.05), but their serum HDL-C level was higher and they had dose dependency. These changes lasted to the end of experiment. (2) In the 11^th week, it was observed the data of 3 therapeutic groups were much the same as that in preventive groups .Things went the similar way between the medium dose therapeutic group and the positive control group, while the high dose therapeutic group gave a better sign than the positive control group. Anyway, no remarkable differences were shown in both of them as compared to positive control group (p>0.05). (3) In the eleventh week, liver index, hepatic TC,TG and MDA level in preventive groups, therapeutic groups and positive group were significantly lower than in high-fat control group (p<0.05), and they had also dose dependency. What's more, in high-fat control group severe liver fatty degeneration was found, while the pathological symptom of the rats in prevention group and treatment group had been fed silkworm pupal unsaturated fatty acids improved markedly. Conclusion: (1) Silkworm pupa oil can reduce serum lipids and prevent the formation of nonalcoholic fatty liver obviously, and they are dose-dependent. The silkworm pupal oil's effect of reducing serum lipids was not markedly different from the scabish oil, but it had better effect on preventing the formation of nonalcoholic fatty liver. (2) Comparing the data of preventive groups with that of therapeutic groups, it could be concluded the preventive groups received a better effect on reducing serum lipids than in therapeutic groups. Therefore, it is best to take preventive measurements to treat heperlipoidemia.