Water Silibinin-induced Hela Cells Apoptosis And Autophagy Mechanism

Silibinin is a polyphenolic flavonoid isolated from the fruits and seeds of Silybum marianum L., It prevents or reverses liver damage caused by alcohol, pesticides, some poisons, or hepatitis. In our previous study, we found that silibinin protected human malignant melanoma cells (A375-S2 cells) against UV irradation resulting in reduced apoptotic ratio, but silibinin and agonistic antibody against death receptor Fas CHll synergistically induced A375-S2 apoptosis. Therefore, silibinin might have bi-directional regulatory effects on induction of apoptosis. In this study, we investigated silibinin induced and augmented cell death of human cervical cancer, HeLa.The studies demonstrate that silibinin inhibited the proliferation of HeLa cells in a time- and dose-dependent manner. MTT assay, photomicroscopical and fluorescence microscopical observation showed that silibinin induced cell apoptosis in HeLa. After treatment with 80μM silibinin, procaspase-8, procaspase-9 and procaspase-3 decreased followed by the degradation of caspase-3 substrates, ICAD (inhibitor of caspase activated DNase), indicating caspase-3 activation. Silibinin also increased expression of pro-apoptotic proteins, FasL, FADD, Bax, Cyto c, and decreased the expression of anti-apoptotic proteins, Bcl-2 and SIRT1. Meanwhile, silibinin induced autophagy antagonized apoptosis in HeLa cells...